Mutagenesis of Trichoderma reesei endoglucanase I: impact of expression host on activity and stability at elevated temperatures

Harshal A Chokhawala; Christine M Roche; Tae-Wan Kim; Meera E Atreya; Neeraja Vegesna; Craig M Dana; Harvey W Blanch; Douglas S Clark

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Mutagenesis of Trichoderma reesei endoglucanase I: impact of expression host on activity and stability at elevated temperatures

机译：里氏木霉内切葡聚糖酶I的诱变：表达宿主对高温下活性和稳定性的影响

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Background Trichoderma reesei is a key cellulase source for economically saccharifying cellulosic biomass for the production of biofuels. Lignocellulose hydrolysis at temperatures above the optimum temperature of T. reesei cellulases (~50°C) could provide many significant advantages, including reduced viscosity at high-solids loadings, lower risk of microbial contamination during saccharification, greater compatibility with high-temperature biomass pretreatment, and faster rates of hydrolysis. These potential advantages motivate efforts to engineer T. reesei cellulases that can hydrolyze lignocellulose at temperatures ranging from 60–70°C.

机译：背景技术里氏木霉是用于经济地糖化纤维素生物质以生产生物燃料的关键纤维素酶来源。在高于里氏木霉纤维素酶最佳温度（〜50°C）的温度下木质纤维素水解可提供许多显着优势，包括在高固体含量下粘度降低，糖化过程中微生物污染的风险降低，与高温生物质预处理的相容性更高，并且水解速度更快。这些潜在的优势促使人们努力设计可在60-70°C的温度下水解木质纤维素的里氏木霉纤维素酶。

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《BMC Biotechnology》 |2015年第1期|共页
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Harshal A Chokhawala; Christine M Roche; Tae-Wan Kim; Meera E Atreya; Neeraja Vegesna; Craig M Dana; Harvey W Blanch; Douglas S Clark;
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中图分类生物工程学（生物技术）;
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1. Disulfide bonds elimination of endoglucanase II from Trichoderma reesei by site-directed mutagenesis to improve enzyme activity and thermal stability: An experimental and theoretical approach [J] . Akbarzadeh Ali, Pourzardosht Navid, Dehnavi Ehsan, International Journal of Biological Macromolecules: Structure, Function and Interactions . 2018,第Pta2期

机译：通过定点诱变从Trichoderma Reesei消除二硫键消除内切葡聚糖酶II，以改善酶活性和热稳定性：一种实验和理论方法
2. The role of the site 342 in catalytic efficiency and pH optima of endoglucanase II from Trichoderma reesei as probed by saturation mutagenesis [J] . YUQI QIN, XIAOMIN WEI, XIN SONG, Biocatalysis and Biotransformation . 2008,第5期

机译：饱和诱变探查342位点在里氏木霉内切葡聚糖酶II催化效率和最佳pH值中的作用
3. Identification of active site carboxylic residues in Trichoderma reesei endoglucanase Cell2A by site-directed mutagenesis [J] . Hirofumi Okada, Kazuki Mori, Kohji Tada, Journal of molecular catalysis, B. Enzymatic . 2000,第1a3期

机译：通过定点诱变鉴定里氏木霉内切葡聚糖酶Cell2A中的活性位点羧基残基
4. Identification of Two Tryptophan Residues in Endoglucanase III from Trichoderma reesei Essential for Cellulose Binding and Catalytic Activity [C] . Ricardo Macarron, Bernard Henrissat, Jozef van Beeuman, American Chemical Society . 1995

机译：纤维素结合和催化活性的Trichogerma Reesei中内葡聚糖酶III中两种色氨酸残留物的鉴定
5. Production of recombinant Trichoderma reesei endoglucanase protein Cel7B by using Kluyveromyces lactis [D] . Alshoug, Zainab Ibrahim 2013

机译：用乳酸克鲁维酵母生产里氏木霉内切葡聚糖酶蛋白Cel7B
6. Mutagenesis of Trichoderma reesei endoglucanase I: impact of expression host on activity and stability at elevated temperatures [O] . Harshal A Chokhawala, Christine M Roche, Tae-Wan Kim, 2015

机译：里氏木霉内切葡聚糖酶I的诱变：表达宿主对高温下活性和稳定性的影响
7. Mutagenesis of Trichoderma reesei endoglucanase I: impact of expression host on activity and stability at elevated temperatures [O] . Harshal A Chokhawala, Christine M Roche, Tae-Wan Kim, 2015

机译：里氏木霉内切葡聚糖酶I的诱变：表达宿主对高温下活性和稳定性的影响
8. Cellulose hydrolysis by Trichoderma reesei cellulases: studies on adsorption, sugar production and synergism of cellobiohydrolase I,II and endoglucanase II [R] . Medve, J. 1997

机译：里氏木霉纤维素酶水解纤维素：纤维二糖水解酶I，II和内切葡聚糖酶II的吸附，糖产生和协同作用的研究

Mutagenesis of Trichoderma reesei endoglucanase I: impact of expression host on activity and stability at elevated temperatures

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