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首页> 外文期刊>Biotechnology for Biofuels >An oleaginous endophyte Bacillus subtilis HB1310 isolated from thin-shelled walnut and its utilization of cotton stalk hydrolysate for lipid production
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An oleaginous endophyte Bacillus subtilis HB1310 isolated from thin-shelled walnut and its utilization of cotton stalk hydrolysate for lipid production

机译：从薄壳核桃中分离出的一种油性内生枯草芽孢杆菌HB1310及其在棉秆水解产物生产脂质中的应用

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Background Third generation biodiesel processing from microbial lipids using low-cost lignocellulosic feedstocks has attracted much attention. Endophytes isolated from oleaginous plants possibly have the capacity to accumulate lipids similar to the hosts. However, little work has been reported in terms of endophytic bacteria isolation from oleaginous plants and their lipid production using lignocellulosic hydrolysate as substrate. Results A new oleaginous endophyte HB1310 has been isolated from the thin-shelled walnut, and identified as Bacillus subtilis on the basis of both 16S rDNA gene sequencing and examination of its physiological and biochemical properties. This strain effectively accumulates cellular lipids using cotton stalk hydrolysate as a substrate. The optimum C/N ratio, culture temperature, and pH value were determined to be 50/1, 30°C, and 6.5, respectively. Batch fermentation was conducted in a bioreactor using these parameters. Satisfactory production, with a maximum lipid productivity of 2.3 g/L, lipid content of 39.8% (w/w), and cell dry weight (CDW) of 5.7 g/L, was obtained at a culture time of 48 h. Variations in the fluorescent intensity and lipid inclusion formation of cells collected at different sampling times illustrate the potential of this bacterium to be useful for cellular lipid production. The fatty acid profile of the produced bacterial lipids showed that the major constituents are myristic, palmitic, stearic, oleic, and linoleic acids with an estimated cetane number of about 61.8, indicating that this strain may be suitable for biodiesel production. Conclusions The present investigation is the first report of an oleaginous endophytic bacterium isolated from the thin-shelled walnut. This strain is capable of producing high lipid contents rapidly using cotton stalk hydrolysate as a substrate, and its lipids are suitable for use as the feedstock for biodiesel production.
机译：背景技术使用低成本木质纤维素原料从微生物脂质加工第三代生物柴油已引起了广泛关注。从油性植物中分离出来的内生菌可能具有与宿主相似的积累脂质的能力。然而，关于从油质植物中分离内生细菌及其使用木质纤维素水解产物作为底物的脂质生产方面，几乎没有报道。结果从薄壳核桃中分离出一种新的含油内生菌HB1310，通过16S rDNA基因测序和对其生理生化特性的检验，鉴定为枯草芽孢杆菌。使用棉秆水解产物作为底物，该菌株有效地积累细胞脂质。最佳C / N比，培养温度和pH值分别确定为50 / 1、30°C和6.5。使用这些参数在生物反应器中进行分批发酵。在48小时的培养时间内获得了令人满意的产量，最大脂质生产率为2.3 g / L，脂质含量为39.8％（w / w），细胞干重（CDW）为5.7 g / L。在不同采样时间收集的细胞的荧光强度和脂质包涵体形成的变化说明了这种细菌可用于细胞脂质生产的潜力。产生的细菌脂质的脂肪酸谱表明，主要成分是肉豆蔻酸，棕榈酸，硬脂酸，油酸和亚油酸，十六烷值估计约为61.8，表明该菌株可能适用于生物柴油的生产。结论本研究是从薄壳核桃中分离出的一种含油内生细菌的首次报道。使用棉秆水解产物作为底物，该菌株能够快速产生高脂质含量，并且其脂质适合用作生物柴油生产的原料。

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