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首页> 外文期刊>Biotechnology for Biofuels >Application of a Burkholderia cepacia lipase-immobilized silica monolith to batch and continuous biodiesel production with a stoichiometric mixture of methanol and crude Jatropha oil
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Application of a Burkholderia cepacia lipase-immobilized silica monolith to batch and continuous biodiesel production with a stoichiometric mixture of methanol and crude Jatropha oil

机译：伯克霍尔德氏菌脂肪酶固定化二氧化硅整体料在甲醇和粗麻风树油化学计量混合物的批量生产和连续生物柴油生产中的应用

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Background The enzymatic production of biodiesel through alcoholysis of triglycerides has become more attractive because it shows potential in overcoming the drawbacks of chemical processes. In this study, we investigate the production of biodiesel from crude, non-edible Jatropha oil and methanol to characterize Burkholderia cepacia lipase immobilized in an n-butyl-substituted hydrophobic silica monolith. We also evaluate the performance of a lipase-immobilized silica monolith bioreactor in the continuous production of biodiesel. Results The Jatropha oil used contained 18% free fatty acids, which is problematic in a base-catalyzed process. In the lipase-catalyzed reaction, the presence of free fatty acids made the reaction mixture homogeneous and allowed bioconversion to proceed to 90% biodiesel yield after a 12 hour reaction time. The optimal molar ratio of methanol to oil was 3.3 to 3.5 parts methanol to one part oil, with water content of 0.6% (w/w). Further experiments revealed that B. cepacia lipase immobilized in hydrophobic silicates was sufficiently tolerant to methanol, and glycerol adsorbed on the support disturbed the reaction to some extent in the present reaction system. The continuous production of biodiesel was performed at steady state using a lipase-immobilized silica monolith bioreactor loaded with 1.67 g of lipase. The yield of 95% was reached at a flow rate of 0.6 mL/h, although the performance of the continuous bioreactor was somewhat below that predicted from the batch reactor. The bioreactor was operated successfully for almost 50 days with 80% retention of the initial yield. Conclusions The presence of free fatty acids originally contained in Jatropha oil improved the reaction efficiency of the biodiesel production. A combination of B. cepacia lipase and its immobilization support, n-butyl-substituted silica monolith, was effective in the production of biodiesel. This procedure is easily applicable to the design of a continuous flow-through bioreactor system.
机译：背景技术通过甘油三酸酯的醇解来酶促生产生物柴油变得更具吸引力，因为它显示出克服化学过程缺陷的潜力。在这项研究中，我们调查了由粗制，不可食用的麻风树油和甲醇生产的生物柴油，以表征固定在正丁基取代的疏水二氧化硅整体中的洋葱伯克霍尔德菌脂肪酶。我们还评估了固定化脂肪酶的二氧化硅整体生物反应器在生物柴油连续生产中的性能。结果所用的麻风树油含有18％的游离脂肪酸，这在碱催化过程中是有问题的。在脂肪酶催化的反应中，游离脂肪酸的存在使反应混合物均匀，并在12小时的反应时间后使生物转化进行到90％的生物柴油收率。甲醇与油的最佳摩尔比为3.3：3.5：甲醇与1：油，水含量为0.6％（w / w）。进一步的实验表明，固定在疏水硅酸盐中的洋葱伯克霍尔德氏菌脂肪酶对甲醇具有足够的耐受性，并且在本反应体系中，吸附在载体上的甘油在某种程度上干扰了反应。使用负载有1.67 g脂肪酶的固定化脂肪酶的二氧化硅整体生物反应器，在稳态下连续生产生物柴油。尽管连续生物反应器的性能略低于分批反应器的预测值，但在0.6 mL / h的流速下仍可达到95％的收率。该生物反应器成功运行了近50天，保留了初始收率的80％。结论麻风树油中最初包含的游离脂肪酸的存在提高了生物柴油生产的反应效率。洋葱头孢杆菌脂肪酶及其固定化支持物正丁基取代的二氧化硅整体料的组合可有效地生产生物柴油。该程序很容易适用于连续流通式生物反应器系统的设计。

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