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首页> 外文期刊>BMC Biotechnology >Immobilized Rhizopus oryzae lipase catalyzed synthesis of palm stearin and cetyl alcohol wax esters: Optimization by Response Surface Methodology
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Immobilized Rhizopus oryzae lipase catalyzed synthesis of palm stearin and cetyl alcohol wax esters: Optimization by Response Surface Methodology

机译：固定化米根霉脂肪酶催化棕榈硬脂精和鲸蜡醇蜡酯的合成：通过响应面法进行优化

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Background Waxes are esters of long-chain fatty acids and long-chain alcohols. Their principal natural sources are animals (sperm whale oil) and vegetables (jojoba) which are expensive and not easily available. Wax esters synthesized by enzymatic transesterification, using palm stearin as raw material, can be considered as an alternative to natural ones. Results Palm stearin is a solid fraction obtained by fractionation of palm oil. Palm stearin was esterified with cetyl alcohol to produce a mixture of wax esters. A non-commercial immobilized lipase from Rhizopus oryzae was used as biocatalyst. Response surface methodology was employed to determine the effects of the temperature (30-50°C), the enzyme concentration (33.34-300 IU/mL), the alcohol/palm stearin molar ratio (3-7 mol/mol) and the substrate concentration (0.06-0.34 g/mL) on the conversion yield of palm stearin. Under optimal conditions (temperature, 30°C; enzyme concentration, 300 IU/mL; molar ratio 3 and substrate concentration 0.21 g/mL) a high conversion yield of 98.52% was reached within a reaction time of 2 h. Conclusions Response surface methodology was successfully applied to determine the optimum operational conditions for synthesis of palm stearin based wax esters. This study may provide useful tools to develop economical and efficient processes for the synthesis of wax esters.
机译：背景蜡是长链脂肪酸和长链醇的酯。它们的主要天然来源是动物（抹香鲸油）和蔬菜（荷荷巴油），它们昂贵且不易获得。以棕榈硬脂为原料，通过酶促酯交换反应合成的蜡酯可被视为天然酯的替代品。结果棕榈硬脂精是通过分馏棕榈油获得的固体级分。棕榈硬脂精用鲸蜡醇酯化以产生蜡酯的混合物。来自米根霉的非商业固定化脂肪酶被用作生物催化剂。使用响应表面方法确定温度（30-50°C），酶浓度（33.34-300 IU / mL），醇/棕榈硬脂精摩尔比（3-7 mol / mol）和底物的影响浓度（0.06-0.34 g / mL）对棕榈硬脂精转化率的影响。在最佳条件下（温度为30°C;酶浓度为300 IU / mL;摩尔比3，底物浓度为0.21 g / mL），在2小时的反应时间内可达到98.52％的高转化率。结论响应面分析法已成功用于确定合成棕榈硬脂基蜡酯的最佳操作条件。这项研究可提供有用的工具，以开发经济有效的蜡酯合成方法。

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