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首页> 外文期刊>BMC Biotechnology >Donor age affects proteome composition of tenocyte-derived engineered tendon
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Donor age affects proteome composition of tenocyte-derived engineered tendon

机译：供体年龄影响肌腱细胞工程化肌腱的蛋白质组组成

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BackgroundThe concept of tissue engineering is to deliver to the injury site biological scaffolds carrying functional cells that will enhance healing response. The preferred cell source is autologous in order to reduce immune response in the treated individual. However, in elderly patients age-related changes in synthetic activity of the implanted cells and subsequent alterations in tissue protein content may affect therapeutic outcomes. In this study we investigated the effect of donor age on proteome composition of tenocyte-derived tendon tissue-engineered constructs. ResultsLiquid chromatography tandem mass spectrometry was used to assess the proteome of tissue-engineered constructs derived from young and old equine tenocytes. Ageing was associated with altered extracellular matrix composition, especially accumulation of collagens (type I, III and XIV), and lower cytoskeletal turnover. Proteins involved in cell responsiveness to mechanical stimuli and cell-extracellular matrix interaction (calponin 1, palladin, caldesmon 1, cortactin) were affected. ConclusionsThis study demonstrated significant changes in proteome of engineered tendon derived from young and old tenocytes, indicating the impact of donor age on composition of autologous constructs.
机译：背景技术组织工程学的概念是将携带功能细胞的生物支架递送至损伤部位，所述功能细胞将增强愈合反应。优选的细胞来源是自体的，以减少所治疗个体的免疫应答。但是，在老年患者中，与年龄相关的植入细胞合成活性的变化以及组织蛋白含量的随后变化可能会影响治疗效果。在这项研究中，我们调查了供体年龄对肌腱细胞腱组织工程构建的蛋白质组学组成的影响。结果采用液相色谱串联质谱法评估了来自年轻和老马肌腱细胞的组织工程构建体的蛋白质组。衰老与改变的细胞外基质组成有关，尤其是胶原蛋白（I，III和XIV型）的积累和较低的细胞骨架更新。涉及细胞对机械刺激和细胞-细胞外基质相互作用的蛋白（钙蛋白1，帕拉丁，钙蛋白1，皮质激素）受到影响。结论这项研究证明了来自年轻和老龄肌腱细胞的工程化肌腱蛋白质组的显着变化，表明供体年龄对自体构建体组成的影响。

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