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首页> 外文期刊>ACS applied materials & interfaces >Book-Shaped Acellular Fibrocartilage Scaffold with Cell-loading Capability and Chondrogenic Inducibility for Tissue-Engineered Fibrocartilage and Bone-Tendon Healing
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Book-Shaped Acellular Fibrocartilage Scaffold with Cell-loading Capability and Chondrogenic Inducibility for Tissue-Engineered Fibrocartilage and Bone-Tendon Healing

机译：书形无细胞纤维纤维率支架具有细胞 - 载能力和组织工程纤维纤维和骨骼愈合的软骨内诱导性

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Functional fibrocartilage regeneration is a bottleneck during bone-tendon healing, and the currently available tissue-engineering strategies for fibrocartilage regeneration are insufficient because of a lack of appropriate scaffold that can load large seeding-cells and induce chondrogenesis of stem cells. The acellular fibrocartilage scaffold (AFS) contains active growth factors as well as tissue-specific epitopes for cell matrix interactions, which make it a potential scaffold for tissue-engineered fibrocartilage. A limitation to this scaffold is that its low porosity inhibits cells loading and infiltration. Here, inspired by book appearance, we sectioned native fibrocartilage tissue (NFT) into book-shape to improve cells loading and infiltration, and then decellularized with four protocols: (1) 2% SDS for 6-h, (2) 2% SDS for 24-h, (3) 4 SDS for 6-h, (4) 4% SDS for 24-h, followed by nuclease digestion. The optimal protocol was screened with respect to microstructures, DNA residence, native ingredients reservation, and chondrogenic inducibility of the AFS. In vitro studies demonstrated that this screened scaffold is noncytotoxicity and lowimmunogenicity, allows adipose-derived stromal cells (ASCs) attachment and proliferation, shows superior chondrogenic inducibility, and stimulates collagen or glycosaminoglycans secretion. The underlying mechanism for this chondrogenic inducibility may be related to hedgehog pathway activating. Additionally, a novel pattern for fabricating tissue-engineered fibrocartilage was developed to enlarge seeding-cells loading, namely, cell-sheets sandwiched by book-shaped scaffold. In-vivo studies indicate that this screened scaffold alone could induce endogenous cells to satisfactorily regenerate fibrocartilage at 16 week, as characterized by fibrocartilaginous extracellular matrix (ECM) deposition and good interface integration. Interleaving this book-shaped AFS with autologous ASCs-sheets significantly enhanced its ability to regenerate fibrocartilage. Cell tracking demonstrated that fibrochondrocytes, osteoblasts, and osteocytes in the healing interface at postoperative 8-week partly originated from the sandwiched ASCs-sheets. On that basis, we propose the use of this book-shaped AFS and cell sheet technique for fabricating tissue-engineered fibrocartilage to improve bone-tendon healing.
机译：功能性纤维纤维测量再生是骨骼肌腱愈合期间的瓶颈，并且目前可用的组织 - 工程策略因缺乏可负荷的适当支架，可以负载大播种细胞并诱导干细胞的软骨组织。细胞纤维纤维轴支架（AFS）含有活性生长因子以及用于细胞基质相互作用的组织特异性表位，这使其成为组织工程纤维纤维化的潜在支架。对该支架的限制是其低孔隙率抑制细胞负载和渗透。在这里，通过书籍外观的启发，我们将天然纤维纤维组织（NFT）分成书形，以改善细胞载荷和渗透，然后用四种方案脱节：（1）2％SDS 6-H，（2）2％SDS对于24-H，（3）4 SDS 6-H，（4）4％SDS 24-H，其次是核酸酶消化。相对于微观结构，DNA住宅，天然成分预留以及AFS的软骨性诱导性筛选最佳方案。体外研究表明，这种筛选的支架是非胞菌毒性和低因素，允许脂肪衍生的基质细胞（ASCS）附着和增殖，显示出优异的软骨形成型诱导性，并刺激胶原或糖氨基聚糖的分泌。这种软骨形成型诱导性的潜在机制可能与刺猬途径有关。另外，开发了一种用于制造组织工程化纤维纤维的新型模式以扩大播种细胞负载，即由书形支架夹在中间的细胞片。体内研究表明，仅通过纤维葡萄球细胞外基质（ECM）沉积和良好的界面集成，仅通过纤维纤维细胞外基质（ECM）沉积和良好的界面整合来诱导内源细胞以令人满意地再生纤维纤维。用自体ASCS-纸张交织这种书形AFS显着提高了其再生纤维纤维的能力。细胞跟踪证明术后8周愈合界面中的纤维素母细胞，成骨细胞和骨细胞部分起源于夹层的ASCS片。在此基础上，我们提出了这种书形AFS和细胞板技术，用于制造组织工程纤维纤维，以改善骨骼肌腱愈合。

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