• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Biotechnology >Aggravation of cold-induced injury in Vero-B4 cells by RPMI 1640 medium – Identification of the responsible medium components
【24h】

Aggravation of cold-induced injury in Vero-B4 cells by RPMI 1640 medium – Identification of the responsible medium components

机译：RPMI 1640培养基加重了Vero-B4细胞的冷诱导损伤–确定负责任的培养基成分

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background In modern biotechnology, there is a need for pausing cell lines by cold storage to adapt large-scale cell cultures to the variable demand for their products. We compared various cell culture media/solutions for cold storage of Vero-B4 kidney cells, a cell line widely used in biotechnology. Results Cold storage in RPMI 1640 medium, a recommended cell culture medium for Vero-B4 cells, surprisingly, strongly enhanced cold-induced cell injury in these cells in comparison to cold storage in Krebs-Henseleit buffer or other cell culture media (DMEM, L-15 and M199). Manufacturer, batch, medium supplements and the most likely components with concentrations outside the range of the other media/solutions (vitamin B12, inositol, biotin, p-aminobenzoic acid) did not cause this aggravation of cold-induced injury in RPMI 1640. However, a modified Krebs-Henseleit buffer with a low calcium concentration (0.42 mM), a high concentration of inorganic phosphate (5.6 mM), and glucose (11.1 mM; i.e. concentrations as in RPMI 1640) evoked a cell injury and loss of metabolic function corresponding to that observed in RPMI 1640. Deferoxamine improved cell survival and preserved metabolic function in modified Krebs-Henseleit buffer as well as in RPMI 1640. Similar Ca2+ and phosphate concentrations did not increase cold-induced cell injury in the kidney cell line LLC-PK1, porcine aortic endothelial cells or rat hepatocytes. However, more extreme conditions (Ca2+ was nominally absent and phosphate concentration raised to 25 mM as in the organ preservation solution University of Wisconsin solution) also increased cold-induced injury in rat hepatocytes and porcine aortic endothelial cells. Conclusion These data suggest that the combination of low calcium and high phosphate concentrations in the presence of glucose enhances cold-induced, iron-dependent injury drastically in Vero-B4 cells, and that a tendency for this pathomechanism also exists in other cell types.
机译：背景技术在现代生物技术中，需要通过冷藏来暂停细胞系以使大规模细胞培养适应其产品的可变需求。我们比较了多种细胞培养基/溶液对Vero-B4肾细胞（一种在生物技术中广泛使用的细胞系）的冷藏能力。结果与在Krebs-Henseleit缓冲液或其他细胞培养基（DMEM，L）中冷藏相比，在Vermi-B4细胞的推荐细胞培养基RPMI 1640培养基中冷藏明显增强了这些细胞的冷诱导细胞损伤。 -15和M199）。生产商，批次，培养基补充剂和最可能的成分，其浓度超出其他培养基/溶液的范围（维生素B 12 ，肌醇，生物素，对氨基苯甲酸酸）不会在RPMI 1640中引起这种冷诱导损伤的恶化。但是，改良的Krebs-Henseleit缓冲液的钙浓度低（0.42 mM），无机磷酸盐浓度高（5.6 mM）和葡萄糖（11.1 mM） ;如在RPMI 1640中的浓度）引起细胞损伤和相应于RPMI 1640中观察到的代谢功能丧失。去铁胺在改良的Krebs-Henseleit缓冲液以及RPMI 1640中提高了细胞存活率并保留了代谢功能。 class =“ a-plus-plus”> 2 + 和磷酸盐浓度并没有增加肾细胞LLC-PK 1 sub>，猪主动脉内皮细胞或大鼠肝细胞。但是，更极端的条件（如在器官保存溶液，威斯康星大学溶液中那样，名义上不存在Ca 2 + 且磷酸盐浓度提高到25 mM）也增加了诱导大鼠肝细胞和猪主动脉内皮细胞的损伤。结论这些数据表明在葡萄糖存在下低钙和高磷酸盐浓度的组合可大大增强Vero-B4细胞的冷诱导铁依赖性损伤，其他类型的细胞也存在这种发病机制的趋势。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号