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首页> 外文期刊>BMC Medical Genomics >Development of a novel DNA sequencing method not only for hepatitis B virus genotyping but also for drug resistant mutation detection
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Development of a novel DNA sequencing method not only for hepatitis B virus genotyping but also for drug resistant mutation detection

机译：不仅可以用于乙型肝炎病毒基因分型，而且可以用于耐药突变检测的新型DNA测序方法的开发

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Background In HBV-infected patients, different genotypes of the hepatitis B virus influence liver disease progression and response to antiviral therapy. Moreover, long-term antiviral therapy will eventually select for drug-resistant mutants. Detection of mutations associated to antiviral therapy and HBV genotyping are essential for monitoring treatment of chronic hepatitis B patients. Results In this study, a simple method of partial-S gene sequencing using a common PCR amplification was established for genotyping clinical HBV isolates sensitively, which could detect the drug-resistant mutations successfully at the same time. Conclusions The partial S gene sequencing assay developed in this study has potential for application in HBV genotyping and drug resistant mutation detection. It is simpler and more convenient than traditional S gene sequencing, but has nearly the same sensitivity and specificity when compared to S gene sequencing.
机译：背景技术在感染了HBV的患者中，不同基因型的乙型肝炎病毒会影响肝脏疾病的进展以及对抗病毒治疗的反应。此外，长期的抗病毒治疗最终将选择耐药突变体。与抗病毒治疗和HBV基因分型相关的突变检测对于监测慢性乙型肝炎患者的治疗至关重要。结果在本研究中，建立了一种使用普通PCR扩增进行S部分基因测序的简单方法，用于敏感地对临床HBV分离株进行基因分型，从而可以成功地检测出耐药突变。结论本研究开发的S基因部分测序方法可用于HBV基因分型和耐药突变检测。它比传统的S基因测序更简单，更方便，但与S基因测序相比具有几乎相同的敏感性和特异性。

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