• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Medical Genomics >Comparing the old and new generation SELDI-TOF MS: implications for serum protein profiling
【24h】

Comparing the old and new generation SELDI-TOF MS: implications for serum protein profiling

机译：比较旧的和新一代的SELDI-TOF MS：对血清蛋白谱分析的影响

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Although the PBS-IIc SELDI-TOF MS apparatus has been extensively used in the search for better biomarkers, issues have been raised concerning the semi-quantitative nature of the technique and its reproducibility. To overcome these limitations, a new SELDI-TOF MS instrument has been introduced: the PCS 4000 series. Changes in this apparatus compared to the older one are a.o. an increased dynamic range of the detector, an adjusted configuration of the detector sensitivity, a raster scan that ensures more complete desorption coverage and an improved detector attenuation mechanism. In the current study, we evaluated the performance of the old PBS-IIc and new PCS 4000 series generation SELDI-TOF MS apparatus. Methods To this end, two different sample sets were profiled after which the same ProteinChip arrays were analysed successively by both instruments. Generated spectra were analysed by the associated software packages. The performance of both instruments was evaluated by assessment of the number of peaks detected in the two sample sets, the biomarker potential and reproducibility of generated peak clusters, and the number of peaks detected following serum fractionation. Results We could not confirm the claimed improved performance of the new PCS 4000 instrument, as assessed by the number of peaks detected, the biomarker potential and the reproducibility. However, the PCS 4000 instrument did prove to be of superior performance in peak detection following profiling of serum fractions. Conclusion As serum fractionation facilitates detection of low abundant proteins through reduction of the dynamic range of serum proteins, it is now increasingly applied in the search for new potential biomarkers. Hence, although the new PCS 4000 instrument did not differ from the old PBS-IIc apparatus in the analysis of crude serum, its superior performance after serum fractionation does hold promise for improved biomarker detection and identification.
机译：背景技术尽管PBS-IIc SELDI-TOF MS装置已广泛用于寻找更好的生物标记物，但有关该技术的半定量性质及其可重复性已经引起了人们的关注。为了克服这些限制，引入了新的SELDI-TOF MS仪器：PCS 4000系列。与较旧的设备相比，该设备的变化是明显的。增加了检测器的动态范围，调整了检测器的灵敏度，确保更完整的解吸覆盖范围的光栅扫描以及改进的检测器衰减机制。在当前的研究中，我们评估了旧的PBS-IIc和新的PCS 4000系列的SELDI-TOF MS设备的性能。方法为此，对两个不同的样品组进行了分析，然后通过两种仪器相继分析了相同的ProteinChip阵列。产生的光谱通过相关的软件包进行分析。通过评估两个样品组中检测到的峰数，生成的峰簇的生物标志物电位和重现性以及血清分馏后检测到的峰数来评估两种仪器的性能。结果我们无法证实所声称的新型PCS 4000仪器性能的改善，这是通过检测到的峰数，生物标记物的潜力和可重复性来评估的。但是，事实证明，PCS 4000仪器在对血清组分进行谱分析后，在峰检测中具有出色的性能。结论由于血清分级分离通过降低血清蛋白质的动态范围促进了低丰度蛋白质的检测，因此它现在越来越多地用于寻找新的潜在生物标志物。因此，尽管新的PCS 4000仪器在分析粗血清方面与旧的PBS-IIc设备没有什么不同，但其在血清分级分离后的优越性能确实有望改善生物标志物的检测和鉴定。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号