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首页> 外文期刊>BMC Veterinary Research >Rumen-derived lipopolysaccharide enhances the expression of lingual antimicrobial peptide in mammary glands of dairy cows fed a high-concentrate diet
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Rumen-derived lipopolysaccharide enhances the expression of lingual antimicrobial peptide in mammary glands of dairy cows fed a high-concentrate diet

机译：瘤胃来源的脂多糖可提高高浓度饮食的奶牛乳腺中舌状抗菌肽的表达

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Background Long-term high-concentrate diet (HCD) feeding can cause subacute ruminal acidosis in cows and subsequently trigger systemic inflammatory and immune responses. Therefore, we conducted the present study in which twelve lactating cows installed with ruminal fistula were randomly assigned to the HCD group (forage:concentrate?=?4:6, n =?6) or the low-concentrate diet (LCD) group (forage:concentrate?=?6:4, n =?6) and were fed for 20?weeks. Ruminal fluid, plasma and mammary gland tissue samples were collected at week 20 for analysing lipopolysaccharide (LPS), pro-inflammatory cytokines, and immune relevant gene expression. The aim of this study was to investigate the effect of rumen-derived LPS on lingual antimicrobial peptide (LAP) synthesis and immune responses in mammary glands of lactating cows fed a HCD. Results Compared with the LCD group, the ruminal pH was lower in the HCD group, while LPS concentrations in the rumen, lacteal artery and vein were higher. The expression of LAP, BNBD5, IL-1β, IL-6, IL-8, and TNF–α was enhanced in the HCD group. LAP protein expression was higher in the HCD group than that in the LCD group. The expression of nuclear factor kappa B (NF-кB) did not change, but was activated, as the amounts of phosphorylated NF-kB and phosphorylated inhibitory kBα increased in the HCD group compared with that in the LCD group. Conclusions After long-term HCD feeding, rumen-derived LPS translocated to the blood stream, triggered inflammatory and immune responses and enhanced LAP synthesis via the NF-kB signalling pathway in mammary glands of lactating cows.
机译：背景长期高浓度饮食（HCD）喂养会导致奶牛的瘤胃酸中毒并引起全身性炎症和免疫反应。因此，我们进行了本研究，其中将十二头装有瘤胃瘘的泌乳牛随机分配到HCD组（饲草：浓缩物？=？4：6，n =？6）或低浓度饮食（LCD）组（饲料：浓缩？=？6：4，n =？6），喂食20周。在第20周时收集了瘤胃液，血浆和乳腺组织样本，以分析脂多糖（LPS），促炎性细胞因子和免疫相关基因的表达。这项研究的目的是调查瘤胃来源的LPS对饲喂HCD的泌乳奶牛的舌头抗菌肽（LAP）合成和免疫反应的影响。结果与LCD组相比，HCD组的瘤胃pH值较低，而瘤胃，乳状动脉和静脉的LPS浓度较高。 HCD组中LAP，BNBD5，IL-1β，IL-6，IL-8和TNF-α的表达增强。 HCD组的LAP蛋白表达高于LCD组。 HCD组的磷酸化NF-kB和磷酸化抑制性kBα的量与LCD组相比有所增加，但核因子κB（NF-кB）的表达没有改变，但被激活。结论长期喂食HCD后，瘤胃来源的LPS易位到血流中，通过NF-kB信号通路触发泌乳奶牛的炎症和免疫反应，并增强LAP合成。

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