• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Microbial Pathogenesis >Lipopolysaccharide induces oxidative stress by triggering MAPK and Nrf2 signalling pathways in mammary glands of dairy cows fed a high-concentrate diet
【24h】

Lipopolysaccharide induces oxidative stress by triggering MAPK and Nrf2 signalling pathways in mammary glands of dairy cows fed a high-concentrate diet

机译：脂多糖通过触发MAPK和NRF2信号通路在奶牛奶牛的乳腺腺体中触发MAPK和NRF2信号传导途径诱导氧化应激

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The goal of current investigations was to reveal the molecular mechanism triggered through feeding a diet with high-concentrate to dairy cows for subacute ruminal acidosis (SARA) induction and to examine the oxidative stress parameters in their mammary epithelial tissue. In an eighteen-weeks feeding trial, 12 Holstein Friesian cows with a standard weight of 455 +/- 28 kg were evenly divided into two groups and given either a low concentrate (LC, forage to concentrate ratio = 6:4) or a high-concentrate (HC, forage to concentrate ratio = 4:6) diet. A remarkable reduction in ruminal pH also increased ruminal lipopolysaccharide (LPS) concentration that was observed in the high-concentrate group of cows at 4 h post-feeding in the morning. Moreover, reduced milk yield was observed in the HC group. The relative mRNA abundance of glutathione peroxidase (GPX) 1 and 3 and superoxide dismutase (SOD) 1 and 2 were down-regulated in high-concentrate fed animals than in the LC, while mRNA was expressed with no change in the of SOD3 among groups. In addition, genes responsible for oxidative stress e.g., ERK, JNK, and p38 were also showed dramatically high mRNA intensity in HC group. The protein concentration of ERK, pERK, pJNK, with pp38, were up-regulated significantly as JNK & p38 showed no big difference. While Nrf2 and pNrf2 were down-regulated considerably in HC group. The total antioxidant capacity (T-AOC) was significantly decreased but of Malondialdehyde (MDA) concentration was raised in HC group than in LC. We thus proposed that higher levels of endogenous LPS may affect the Mitogen-activated protein kinases (MAPK) and nuclear factor erythroid 2-related factor 2 (Nrf2)-dependent antioxidant response.
机译：目前的调查的目标是揭示通过用高浓缩的奶牛喂养饮食而引发的分子机制，用于亚急性瘤胃酸中毒（SARA）诱导，并检查其乳腺上皮组织中的氧化应激参数。在18周的饲养试验中，标准重量为455 +/- 28公斤的12个荷斯坦弗里斯奶牛均匀分为两组，并给予低浓缩物（LC，饲料浓缩比率= 6：4）或高 - 康流（HC，牧草，浓缩比率= 4：6）饮食。瘤胃pH值的显着降低也增加了在早晨4小时的高浓缩牛奶牛中观察到的瘤胃脂多糖（LPS）浓度增加。此外，在HC组中观察到降低的乳率。谷胱甘肽过氧化物酶（GPX）1和3和超氧化物歧化酶（SOD）1和2的相对mRNA富于高浓缩喂养动物中的比例小于LC，而mRNA在组中没有SOD3没有变化。此外，负责氧化应激的基因例如ERK，JNK和P38在HC组中均显着高mRNA强度。用PP38的ERK，PERK，PJNK的蛋白质浓度显着上调为JNK＆amp; P38没有大的差异。虽然NRF2和PNRF2在HC组中显着下调。总抗氧化能力（T-AOC）显着降低，但在HC组中升高了丙二醛（MDA）浓度而不是LC。因此，我们提出了更高水平的内源性LP可能会影响丝裂原激活的蛋白激酶（MAPK）和核因子红霉菌2相关因子2（NRF2） - 依赖性抗氧化反应。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号