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首页> 外文期刊>BMC Pulmonary Medicine >Circulating microRNAs as biomarkers for Sepsis secondary to pneumonia diagnosed via Sepsis 3.0
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Circulating microRNAs as biomarkers for Sepsis secondary to pneumonia diagnosed via Sepsis 3.0

机译：循环microRNAs作为通过脓毒症3.0诊断出的继发于肺炎的脓毒症的生物标志物

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Sepsis biomarkers have limited specificity and sensitivity. Few studies have investigated microRNA (miRNA) biomarkers for sepsis secondary to pneumonia. This study aims to investigate the diagnostic and prognostic values of miRNAs in sepsis secondary to pneumonia. Sepsis 3.0 was used to diagnose sepsis. Screening was performed through the Agilent miRNA chip technology by using the following criteria: p 50 change. This study recruited 52 patients with pneumonia, including 31 males (59.6%) and 21 females (40.4%), 44 patients with sepsis secondary to pneumonia were diagnosed via Sepsis 3.0 (34 [77.3%] males and 10 [22.7%] females), and 21 healthy controls were used for miRNA verification. The miRNA levels were detected through fluorescence real-time quantitative polymerase chain reaction (qRT-PCR). Results: Fluorescence qRT-PCR detection showed that the miR-7110-5p and miR-223-3p expression levels in both patient groups were upregulated compared with those in the healthy controls. The expression levels differed between patients with pneumonia and those with sepsis secondary to pneumonia. The sensitivity and specificity of miR-7110-5p to differentiate sepsis from healthy controls were 84.2 and 90.5%, whereas those of miR-223-3p were 82.9 and 100%, respectively. Multivariate analysis of variance suggested that the presence of sepsis affected the miR-223-3p level (p?=?0.041), whereas the presence of sepsis (p?=?0.000) and the underlying disease (p?=?0.025) influenced the miR-7110-5p level. MiR-223-3p could be utilized to predict sepsis secondary to pneumonia.
机译：败血症生物标志物的特异性和敏感性有限。很少有研究针对肺炎继发性败血症的microRNA（miRNA）生物标志物进行研究。这项研究旨在调查miRNA在肺炎继发性败血症中的诊断和预后价值。脓毒症3.0用于诊断脓毒症。使用以下标准通过安捷伦miRNA芯片技术进行筛选：p 50改变。该研究招募了52例肺炎患者，包括31例男性（59.6％）和21例女性（40.4％），通过败血症3.0诊断出44例继发于肺炎的败血症患者（34例男性[77.3％]和10例[22.7％]女性） ，并使用21个健康对照进行miRNA验证。通过荧光实时定量聚合酶链反应（qRT-PCR）检测miRNA的水平。结果：荧光qRT-PCR检测显示，与健康对照组相比，两组患者的miR-7110-5p和miR-223-3p表达水平均上调。肺炎患者和继发于肺炎的败血症患者之间的表达水平不同。 miR-7110-5p区分败血症和健康对照的敏感性和特异性分别为84.2和90.5％，而miR-223-3p的敏感性和特异性分别为82.9和100％。多变量方差分析表明败血症的存在影响了miR-223-3p水平（p？=？0.041），而脓毒症的存在（p？=？0.000）和基础疾病（p？=？0.025）会产生影响。 miR-7110-5p水平。 MiR-223-3p可用于预测继发于肺炎的败血症。

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