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首页> 外文期刊>BMC Immunology >The imbalance of Th17/Treg cells is involved in the progression of nonalcoholic fatty liver disease in mice
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The imbalance of Th17/Treg cells is involved in the progression of nonalcoholic fatty liver disease in mice

机译：Th17 / Treg细胞的失衡与小鼠非酒精性脂肪肝疾病的进展有关

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Background Non-alcoholic fatty liver disease (NAFLD) is a common, chronic liver disease worldwide. Recent studies have shown that T helper (Th) 17 and regulatory T (Treg) cells play critical roles in various disorders of liver inflammation. Here, we explored the value of polyene phosphatidylcholine capsules (PPC) for regulating the imbalance of Th17/Treg cells in the pathogenesis of mice with NAFLD. Methods C57BL/6 mice were randomly divided into three groups as follows:normal diet (ND), high-fat diet (HF),and HF plus PPC(HF?+?PPC). The frequencies of splenic Th17 and Treg cells were measured by flow cytometry, and their related cytokines were analyzed by CBA and real-time PCR. Results At the end of 24?weeks, mice in the HF group had a higher frequency of intrahepatic Th17 cells,and a lower proportion of Treg cells compared with the ND group. The levels of Th17 cell-related cytokines (IL-6, IL-17 and IL-23) in serum and in liver tisse were increased,and the hepatic mRNA levels of RORγt, STAT3 and IL-6 were also increased. By contrast,the FoxP3 mRNA level was decreased in the HF group. Moreover, significant pathological and biochemical changes in the liver, as well as serum biochemical changes, were found in mice with NAFLD. Interestingly, following treatment with PPC, the levels of liver inflammation,frequencies of Th17/Treg cells and associated cytokines,and biochemical data were significantly altered. Conclusion These findings demonstrate a critical role for PPC in partially attenuating liver inflammatory responses in mice with NAFLD that involves the imbalance of Treg/Th17 cells and associated cytokines.
机译：背景技术非酒精性脂肪肝病（NAFLD）是世界范围内常见的慢性肝病。最近的研究表明，T辅助（Th）17和调节性T（Treg）细胞在各种肝炎疾病中起关键作用。在这里，我们探讨了多烯磷脂酰胆碱胶囊（PPC）在调节NAFLD小鼠发病机理中对Th17 / Treg细胞失衡的价值。方法C57BL / 6小鼠随机分为三组：正常饮食（ND），高脂饮食（HF），HF加PPC（HF + PPC）。通过流式细胞术测量脾脏Th17和Treg细胞的频率，并通过CBA和实时PCR分析它们的相关细胞因子。结果与ND组相比，HF组小鼠肝内Th17细胞频率较高，而Treg细胞比例较低，在24周结束时。血清和肝组织中Th17细胞相关细胞因子（IL-6，IL-17和IL-23）的水平升高，RORγt，STAT3和IL-6的肝mRNA水平也升高。相比之下，HF组的FoxP3 mRNA水平下降。此外，在患有NAFLD的小鼠中发现肝脏的显着病理学和生化变化以及血清生化变化。有趣的是，PPC治疗后，肝脏炎症水平，Th17 / Treg细胞和相关细胞因子的频率以及生化数据发生了显着变化。结论这些发现表明，PPC在部分减轻NAFLD小鼠肝脏炎症反应中起关键作用，涉及Treg / Th17细胞和相关细胞因子的失衡。

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