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首页> 外文期刊>BMC Anesthesiology >Intravenous pretreatment with emulsified isoflurane preconditioning protects kidneys against ischemia/reperfusion injury in rats
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Intravenous pretreatment with emulsified isoflurane preconditioning protects kidneys against ischemia/reperfusion injury in rats

机译：乳化异氟烷预处理静脉内预处理可保护肾脏免受大鼠缺血/再灌注损伤

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Background Emulsified isoflurane (EIso) is a novel intravenous general anesthetic, which can provide rapid anesthetic induction and recovery. EIso preconditioning could attenuate heart, lung and liver ischemia/reperfusion (I/R) injury. We tested the hypothesis that intravenous pretreatment with EIso would protect kidneys against I/R injury by inhibiting systemic inflammatory responses and improving renal antioxidative ability. Methods Rats were randomly divided into these six groups: sham, I/R, intralipid, 1, 2 or 4?ml/kg EIso. Rats were subjected to 45?min left renal pedicle occlusion followed by 3?h reperfusion after right nephrectomy. Rat were treated with intravenous 8% EIso with 1, 2 or 4?ml/kg, or 30% intralipid with 2?ml/kg for 30?min before ischemia, respectively. After reperfusion, renal functional parameters, serum mediator concentrations and markers of oxidative stress in kidney tissues were determined, and renal histopathological analysis were performed. Results Serum creatinine, blood urea nitrogen, cystatin c, tumor necrosis factor-α, interleukin-6, and interleukin-10 concentrations were significantly increased after renal I/R as compared to the sham group. So was renal tissue MDA content and histological scores, but renal tissue SOD activity was decreased. Additionally, severe morphological damages were observed in these study groups. In contrast, 2 or 4?ml/kg EIso reduced serum creatinine, blood urea nitrogen, cystatin c, tumor necrosis factor-α, and interleukin-6 levels, decreased renal tissue MDA content and histological scores, increased serum interleukin-10 level and tissue SOD activity as compared to the I/R, intralipid and 1?ml/kg EIso groups. Renal morphological damages were alleviated after pretreatment of 2 or 4?ml/kg EIso. Conclusions Intravenous EIso produces preconditioning against renal I/R injury in rats, which might be mediated by attenuating inflammation and increasing antioxidation ability.
机译：背景技术乳化异氟烷（EIso）是一种新型的静脉全身麻醉剂，可提供快速的麻醉诱导和恢复。 EIso预处理可以减轻心脏，肺和肝脏的缺血/再灌注（I / R）损伤。我们测试了以下假设：用EIso进行静脉内预处理可通过抑制全身性炎症反应和提高肾脏抗氧化能力来保护肾脏免受I / R损伤。方法将大鼠随机分为假手术，I / R，血脂，1、2或4？ml / kg EIso这六组。大鼠右肾切除后进行45分钟的左肾蒂闭塞，然后再灌注3小时。在缺血前，分别用8％，1、2或4？ml / kg的EIso或30％脂质体的2？ml / kg的30％脂质治疗大鼠30分钟。再灌注后，测定肾组织中的肾功能参数，血清介质浓度和氧化应激标志物，并进行肾脏组织病理学分析。结果与假手术组相比，肾I / R后血清肌酐，血尿素氮，胱抑素C，肿瘤坏死因子-α，白细胞介素6和白细胞介素10的浓度显着升高。肾组织的MDA含量和组织学评分也有所提高，但肾组织的SOD活性却降低了。此外，在这些研究组中观察到严重的形态学损害。相反，EIso 2或4？ml / kg降低了血清肌酐，血尿素氮，胱抑素c，肿瘤坏死因子-α和白细胞介素6的水平，肾组织MDA含量和组织学评分降低，血清白细胞介素10的水平和与I / R，脂质内和1？ml / kg EIso组相比，组织SOD活性更高。预处理2或4？ml / kg EIso后，可以减轻肾脏的形态损害。结论静脉EIso可以减轻大鼠肾脏I / R损伤，可能是通过减轻炎症反应和增加抗氧化能力介导的。

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