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首页> 外文期刊>Brazilian Journal of Infectious Diseases >Epstein-Barr virus nuclear antigen-2 detection and typing in immunocompromised children correlated with lymphoproliferative disorder biopsy findings
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Epstein-Barr virus nuclear antigen-2 detection and typing in immunocompromised children correlated with lymphoproliferative disorder biopsy findings

机译：免疫力低下儿童的爱泼斯坦-巴尔病毒核抗原2检测和分型与淋巴增生性疾病活检结果相关

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Epstein-Barr virus (EBV), the causative agent of infectious mononucleosis, plays a significant role as a cofactor in the process of tumorigenesis, and has consistently been associated with a variety of malignancies especially in immunocompromised patients. Forty-four children and adolescents (21 liver transplant patients, 7 heart transplant, 5 AIDS, 3 autoimmune hepatitis, 2 nephritic syndromes, 2 medullar aplasia, 2 primary immunodeficiency disorder patients, 1 thrombocytopenic purpura and 1 systemic lupus erythematosus) presenting with chronic active EBV infection (VCA-IgM persistently positive; VCA-IgG > 20 AU/mL and positive IgG _ EBNA) had peripheral blood samples obtained during clinically characterized EBV reactivation episodes. DNA samples were amplified in order to detect and type EBV on the basis of the EBNA-2 sequence (EBNA2 protein is essential for EBV-driven immortalization of B lymphocytes). Although we have found a predominance of type 1 EBNA-2 virus (33/44; 75%), 10 patients (22.73%) carried type 2 EBNA-2, and one liver transplant patient (2.27%) a mixture of the two types, the higher proportion of type 2 EBV, as well as the finding of one patient bearing the two types is in agreement with other reports held on lymphoproliferative disorder (LPD) patients, which analyzed tumor biopsies. We conclude that EBNA-2 detection and typing can be performed in peripheral blood samples, and the high prevalence of type 2 in our casuistic indicates that this population is actually at risk of developing LPD, and should be monitored.
机译：传染性单核细胞增多症的病原体爱泼斯坦-巴尔病毒（EBV）在肿瘤发生过程中作为辅助因子发挥着重要作用，并且一直与多种恶性肿瘤相关，尤其是在免疫功能低下的患者中。表现为慢性活动的44名儿童和青少年（21例肝移植患者，7例心脏移植，5例艾滋病，3例自身免疫性肝炎，2例肾病综合征，2例延髓发育不良，2例原发性免疫缺陷疾病患者，1例血小板减少性紫癜和1例系统性红斑狼疮） EBV感染（VCA-IgM持续呈阳性; VCA-IgG> 20 AU / mL，且IgG _ EBNA呈阳性）具有在临床特征性EBV激活发作期间获得的外周血样本。为了根据EBNA-2序列检测EBV并进行分型，对DNA样本进行了扩增（EBNA2蛋白对于EBV驱动的B淋巴细胞永生化至关重要。尽管我们发现1型EBNA-2病毒占多数（33/44; 75％），但10例患者（22.73％）携带2型EBNA-2，而1名肝移植患者（2.27％）则是两种类型的混合物，较高的2型EBV比例，以及一名患有两种类型EBV的患者的发现，与关于淋巴增生性疾病（LPD）患者的其他报告一致，后者分析了肿瘤活检。我们得出的结论是，可以在外周血样本中进行EBNA-2检测和分型，并且在我们的病因中2型患病率很高表明该人群实际上有患LPD的风险，应予以监测。

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