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首页> 外文期刊>Biotropia: the Southeast Asian journal of tropical biology >CYTOTOXIC PROTEIN FROM Trichosanthes cucumerina L. var anguina (L.) Haines
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CYTOTOXIC PROTEIN FROM Trichosanthes cucumerina L. var anguina (L.) Haines

机译：Trichosanthes cucumerina L. var anguina（L.）Haines的细胞毒素蛋白

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Normal 0 false false false IN X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable{mso-style-name:"Table Normal";mso-tstyle-rowband-size:0;mso-tstyle-colband-size:0;mso-style-noshow:yes;mso-style-priority:99;mso-style-qformat:yes;mso-style-parent:"";mso-padding-alt:0cm 5.4pt 0cm 5.4pt;mso-para-margin:0cm;mso-para-margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:11.0pt;font-family:"Calibri","sans-serif";mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:"Times New Roman";mso-fareast-theme-font:minor-fareast;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;} Three proteins were discovered, they were: TF2 from fruit, TS3 from seed and TR3 from root of Trichosanthes cucumerina L. var anguina (L.) Haines by extraction with Phosphate Buffer Saline (PBS). The proteins were precipitated using 80% saturated ammonium sulphate and then were dialysed using cellophane. Furthermore, the dialysate was fractionated through gel filtration chromatography. The highest protein yield was 1.109 % , resulted from TS3, then 0.356% from TR3, and the lowest was 0.014% from TF2. C haracterization of the proteins using SDS-PAGE indicated that a ll of the protein fractions showed more than one sub unit molecular masses (Mr) in the range of 16 - 29 kDa. The toxicity ( LC 5 0 ) of proteins on brine shrimp lethality test were within range of 19 μg/ml - 25 μg/ml . The c ytotoxicities of the TR3 and TS3 proteins on cancer cell line indicated that both of the active protein s inhibit proliferation of HeLa and K-562 cell s with IC 50 up to 45 μ g/ml. Keywords : Trichosanthes cucumerina L. var anguina (L.), plant part, protein, yield, toxicity, cytotoxicity, molecular mass .
机译：正常0否否否IN X-NONE X-NONE MicrosoftInternetExplorer4 / *样式定义* / table.MsoNormalTable {mso-style-name：“ Table Normal”; mso-tstyle-rowband-size：0; mso-tstyle-colband-大小：0; mso-style-noshow：是; mso-style优先级：99; mso-style-qformat：是; mso-style-parent：“”; mso-padding-alt：0cm 5.4pt 0cm 5.4pt; mso-para-margin：0cm; mso-para-margin-bottom：.0001pt; mso-pagination：widow-orphan; font-size：11.0pt; font-family：“ Calibri”，“ sans-serif”; mso- ascii-font-family：Calibri; mso-ascii-theme-font：未成年人; mso-fareast-font-family：“ Times New Roman”; mso-fareast-theme-font：minor-fareast; mso-hansi- font-family：Calibri; mso-hansi-theme-font：minor-latin; mso-bidi-font-family：“ Times New Roman”; mso-bidi-theme-font：minor-bidi;}发现了三种蛋白质，它们是：果实中的TF2，种子中的TS3和瓜cho藜（Trichosanthes cucumerina L. var anguina（L.）Haines）根部的TR3，通过磷酸盐缓冲液（PBS）提取。使用80％饱和硫酸铵沉淀蛋白质，然后使用玻璃纸透析。此外，将透析液通过凝胶过滤色谱分离。 TS3产生的蛋白质最高产量为1.109％，TR3为0.356％，而TF2最低为0.014％。使用SDS-PAGE对蛋白质进行特征化分析表明，其中的11个蛋白质部分在16-29 kDa的范围内显示一个以上的亚单位分子量（Mr）。蛋白质对盐水虾致死性试验的毒性（LC 5 0）在19μg/ ml-25μg/ ml范围内。 TR3和TS3蛋白对癌细胞系的细胞毒性表明，这两种活性蛋白均能抑制HeLa和K-562细胞的增殖，IC 50最高可达45μg / ml。关键字：瓜Tri瓜（Trichosanthes cucumerina L. var anguina，植物部位），蛋白质，产量，毒性，细胞毒性，分子量。

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