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Novel method for evaluation of anaerobic germination in rice and its application to diverse genetic collections

机译:水稻厌氧萌发评估的新方法及其在多种遗传资源中的应用

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Direct seeding saves time and labour in the cultivation of rice. However, seedling establishment is often unstable, and yields are lower than in transplanting. Anaerobic germination (AG) is a key trait for improvement of direct seeding of rice. We established a simple and reliable method of evaluating AG in rice breeding. We germinated seeds in distilled water or deoxygenated water and measured coleoptile length several days later; compared the results of each method with survival rate in flooded soil; and used the anoxic water method for QTL analysis and for testing cultivars. Coleoptile elongation in anoxic water and survival rate in flooded soil were significantly correlated ( r = 0.879, P 0.01). A significant QTL, likely to be a major gene ( AG1 ), was found in chromosome segment substitution lines and in a backcrossed F2 population derived from tolerant and sensitive lines. Diverse rice genetic resources were classified into tolerant or sensitive accession groups reflecting their ecotypes. Our study revealed that anoxic water evaluation method saves space and time in a stable environment compared with flooded soil evaluation. It is applicable to QTL analysis and isolation of genes underlying anaerobic germination.
机译:直接播种节省了水稻种植的时间和劳动力。然而,幼苗的建立通常是不稳定的,并且产量低于移植中的产量。无氧萌发(AG)是改善水稻直接播种的关键性状。我们建立了一种简单可靠的水稻育种中AG评估方法。我们在蒸馏水或脱氧水中使种子发芽,几天后测量胚芽鞘长度。将每种方法的结果与在淹水土壤中的存活率进行比较;并使用缺氧水法进行QTL分析和测试品种。缺氧条件下胚芽鞘的伸长与淹水土壤的存活率之间存在显着相关性(r = 0.879,P <0.01)。在染色体片段替代品系和从耐受和敏感品系衍生的回交F2群体中发现了一个重要的QTL,很可能是主要基因(AG1)。不同的稻米遗传资源被分为反映其生态类型的耐性或敏感种质。我们的研究表明,与淹水土壤评估相比,缺氧水评估方法在稳定的环境中节省了空间和时间。它适用于QTL分析和分离厌氧萌发相关基因。

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