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Novel method for evaluation of anaerobic germination in rice and its application to diverse genetic collections

机译:水稻厌氧萌发的新方法及其在不同遗传收藏中的应用

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Direct seeding saves time and labour in the cultivation of rice. However, seedling establishment is often unstable, and yields are lower than in transplanting. Anaerobic germination (AG) is a key trait for improvement of direct seeding of rice. We established a simple and reliable method of evaluating AG in rice breeding. We germinated seeds in distilled water or deoxygenated water and measured coleoptile length several days later; compared the results of each method with survival rate in flooded soil; and used the anoxic water method for QTL analysis and for testing cultivars. Coleoptile elongation in anoxic water and survival rate in flooded soil were significantly correlated (r = 0.879, P < 0.01). A significant QTL, likely to be a major gene (AG1), was found in chromosome segment substitution lines and in a backcrossed F-2 population derived from tolerant and sensitive lines. Diverse rice genetic resources were classified into tolerant or sensitive accession groups reflecting their ecotypes. Our study revealed that anoxic water evaluation method saves space and time in a stable environment compared with flooded soil evaluation. It is applicable to QTL analysis and isolation of genes underlying anaerobic germination.
机译:直接播种节省水稻种植时间和劳动力。然而,幼苗建立通常不稳定,产量低于移植。厌氧发芽(AG)是改善水稻直接播种的关键特征。我们在水稻育种中建立了一种评价AG的简单可靠方法。我们在蒸馏水或脱氧水中发芽的种子,几天后测量了菌植物长度;将各种方法的结果与淹水土壤中生存率进行了比较;并利用QTL分析和测试品种的缺氧水方法。浸泡土壤中的植物水和存活率的植物精化伸长率明显相关(r = 0.879,p <0.01)。在染色体分段取代线中发现可能是主要基因(AG1)的重要QTL,并且在衍生自耐受性和敏感线的回复的F-2群中发现。不同的水稻遗传资源被分为宽容或敏感的入境团体,反映了他们的生态型。我们的研究表明,与洪水土壤评估相比,缺氧水评估方法在稳定的环境中节省了空间和时间。它适用于厌氧发芽潜在的基因的QTL分析和分离。

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