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首页> 外文期刊>British Biotechnology Journal >In vitro Cytological Studies of Leaf Callus Cultures of Orthosiphon aristatus (Blume) Miq
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In vitro Cytological Studies of Leaf Callus Cultures of Orthosiphon aristatus (Blume) Miq

机译：伞形拟南芥叶片愈伤组织培养的体外细胞学研究

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The study was carried out to unravel the in vitro cytological behaviour of leaf callus cultures of Orthosiphon ariststus (Blume) Miq. The cultures were raised using leaf explants on Murashige and Skoog (MS) medium supplemented with different growth regulators like auxins (2,4-D, IAA, NAA) and cytokinin (BAP) alone and in different concentrations and combinations. Best callusing from the leaf explants was achieved in 2,4-D supplemented MS medium at 5 mg/l concentration. Cytogenetic analysis of the chromosomal variations was carried out for primary cultures as well as 3-month-old callus cultures. The chromosome number did not show variations and the cells studied were observed to be diploid (2n= 28) in nature. Among the numerical observations induced, polyploid cells were the most frequent. The frequency of bridges was observed among the other structural changes. The composition and concentration of the growth regulators was found to affect chromosomal instability with multinucleate, multinucleolate and embryogenic masses. These observations indicate that genetic variations may arise during cell cultures.
机译：进行了这项研究，以揭示米色Orthosiphon ariststus（Blume）Miq的叶片愈伤组织培养物的体外细胞学行为。使用Murashige和Skoog（MS）培养基上的叶外植体培养培养物，并补充不同的生长调节剂，如生长素（2,4-D，IAA，NAA）和细胞分裂素（BAP）单独并以不同的浓度和组合使用。在2,4-D补充MS培养基中，浓度为5 mg / l时，叶片外植体产生了最佳的愈伤组织。对原代培养以及3个月大的愈伤组织培养进行了染色体变异的细胞遗传学分析。染色体数目未显示变异，观察到的细胞本质上是二倍体（2n = 28）。在诱导的数值观察中，多倍体细胞是最常见的。除其他结构变化外，还观察到桥梁的频率。发现生长调节剂的组成和浓度会影响多核，多核苷和胚胎发生质量的染色体不稳定性。这些观察结果表明在细胞培养过程中可能出现遗传变异。

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