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首页> 外文期刊>BMC Microbiology >Identification of a bacteriocin and its cognate immunity factor expressed by Moraxella catarrhalis
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Identification of a bacteriocin and its cognate immunity factor expressed by Moraxella catarrhalis

机译：卡他莫拉菌表达的细菌素及其同源免疫因子的鉴定

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Bacteriocins are antimicrobial proteins and peptides ribosomally synthesized by some bacteria which can effect both intraspecies and interspecies killing. Moraxella catarrhalis strain E22 containing plasmid pLQ510 was shown to inhibit the growth of M. catarrhalis strain O35E. Two genes (mcbA and mcbB) in pLQ510 encoded proteins predicted to be involved in the secretion of a bacteriocin. Immediately downstream from these two genes, a very short ORF (mcbC) encoded a protein which had some homology to double-glycine bacteriocins produced by other bacteria. A second very short ORF (mcbI) immediately downstream from mcbC encoded a protein which had no significant similarity to other proteins in the databases. Cloning and expression of the mcbI gene in M. catarrhalis O35E indicated that this gene encoded the cognate immunity factor. Reverse transcriptase-PCR was used to show that the mcbA, mcbB, mcbC, and mcbI ORFs were transcriptionally linked. This four-gene cluster was subsequently shown to be present in the chromosome of several M. catarrhalis strains including O12E. Inactivation of the mcbA, mcbB, or mcbC ORFs in M. catarrhalis O12E eliminated the ability of this strain to inhibit the growth of M. catarrhalis O35E. In co-culture experiments involving a M. catarrhalis strain containing the mcbABCI locus and one which lacked this locus, the former strain became the predominant member of the culture after overnight growth in broth. This is the first description of a bacteriocin and its cognate immunity factor produced by M. catarrhalis. The killing activity of the McbC protein raises the possibility that it might serve to lyse other M. catarrhalis strains that lack the mcbABCI locus, thereby making their DNA available for lateral gene transfer.
机译：细菌素是一些细菌通过核糖体合成的抗菌蛋白和肽，可以影响种内和种间杀伤。显示含有质粒pLQ510的卡他莫拉氏菌菌株E22抑制卡他莫拉氏菌菌株O35E的生长。 pLQ510中的两个基因（mcbA和mcbB）编码的蛋白预计与细菌素的分泌有关。在这两个基因的下游，一个很短的ORF（mcbC）编码的蛋白质与其他细菌产生的双甘氨酸细菌素具有某些同源性。紧邻mcbC下游的第二个非常短的ORF（mcbI）编码的蛋白质与数据库中的其他蛋白质没有显着相似性。粘膜炎莫拉氏菌O35E中mcbI基因的克隆和表达表明该基因编码同源免疫因子。逆转录PCR-PCR用于显示mcbA，mcbB，mcbC和mcbI ORF转录连接。随后，该四基因簇被证明存在于包括O12E在内的几种粘膜炎莫拉氏菌菌株的染色体中。卡他氏菌O12E中的mcbA，mcbB或mcbC ORF失活消除了该菌株抑制卡他氏菌O35E生长的能力。在涉及含有mcbABCI基因座和缺少该基因座的卡他氏菌菌株的共培养实验中，前一种菌株在肉汤中过夜生长后成为培养物中的主要成员。这是对卡他莫拉氏菌产生的细菌素及其同源免疫因子的首次描述。 McbC蛋白的杀伤活性增加了它可能可以裂解其他缺乏mcbABCI基因座的卡他氏菌菌株的可能性，从而使其DNA可用于侧向基因转移。

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