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首页> 外文期刊>British Biotechnology Journal >Optimization of Cellulase (E.C. 3.2.1: 4)Production Using Penicillium citrinum MTCC9620 in Solid State Fermentation
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Optimization of Cellulase (E.C. 3.2.1: 4)Production Using Penicillium citrinum MTCC9620 in Solid State Fermentation

机译：在固态发酵中使用柠檬青霉MTCC9620优化纤维素酶的生产（E.C. 3.2.1：4）

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Aim: The objective of the present work was to optimize the environmental parameters for Cellulase (1,4 β-endoglucanase, E.C.3.2.1:4) production using Penicillium citrinum MTCC 9620 in Solid State Fermentation.Study Design: One unit of Cellulase (1,4 β-endoglucanase, E.C.3.2.1:4) activity is defined as the amount of enzyme producing 1μmole of glucose equivalent/min measured using UV visible spectrophotometer at 540 nm.Place and Duration of Study: Food Technology laboratory of Dr. S. S. Bhatnagar University Institute of Chemical Engineering & Technology, Panjab University, Chandigarh between January and June 2011.Methodology: Penicillium citrinum MTCC 9620 was maintained on potato dextrose agar (PDA) at 4°C. For Cellulase production Czapek Yeast Extract medium was used as moistening medium. Incubation temperature, pH, incubation time and other parameters like suitable substrate, pre-treatment of the substrate on production of Cellulase in Solid State Fermentation (SSF) was optimized using agricultural residues by Penicillium citrinum MTCC 9620. Microscopic and Spectral properties of substrates were determined to detect the structural changes after pre-treatment.Results: Production of extracellular Cellulase was greatly affected by variation in substrates, pre-treatments of substrate and variation in pH and incubation temperature. Cellulase activity was significantly (p Penicillium citrinum MTCC 9620 after 120 h of fermentation time. SEM study revealed that during alkali treatment the solid surface become rough which results growth of fungus eventually maximum cellulase production.Conclusion: P. citrinum MTCC 9620 is one of the potential cellulase producing fungal strain. Optimum condition of cellulase (1,4 β-endoglucanase, E.C.3.2.1:4) production by P. citrinum MTCC 9620 was 30°C temperature, 5.5 pH when alkali treated wheat bran was used as substrate. Growth kinetics of P. citrinum MTCC 9620 was studied and it showed adequacy of fit to Monod Model to describe the growth pattern of P. citrinum MTCC 9620 in SSF at 30°C for 120 h incubation period.
机译：目的：本研究的目的是优化在固态发酵中使用柠檬青霉MTCC 9620生产纤维素酶（1,4β-内切葡聚糖酶，EC3.2.1：4）的环境参数。研究设计：一单位纤维素酶（ 1,4β-内切葡聚糖酶，EC3.2.1：4）活性定义为使用紫外线可见分光光度计在540 nm下测得的产生1μmol葡萄糖当量/分钟的酶的量。 2011年1月至6月间，印度潘贾布大学（University of Panjab University）的SS Bhatnagar大学化学工程与技术学院。方法：将柠檬青霉MTCC 9620保持在4℃的马铃薯葡萄糖琼脂（PDA）上。为了生产纤维素酶，将Czapek酵母提取物培养基用作润湿培养基。孵育温度，pH，孵育时间和其他参数（如合适的底物，固态发酵中纤维素酶生产中底物的预处理）使用柠檬酸青霉MTCC 9620的农业残留物进行了优化。确定了底物的显微和光谱特性结果：底物的变化，底物的预处理以及pH值和孵育温度的变化极大地影响了细胞外纤维素酶的产生。纤维素酶活性显着（发酵120 h后对柠檬青霉MTCC9620。SEM研究表明，在碱处理过程中，固体表面变得粗糙，最终导致真菌的生长，最终使纤维素酶的产量最高。结论：柠檬青霉MTCC 9620是其中之一）。以碱处理过的麦麸为底物时，柠檬假单胞菌MTCC 9620生产纤维素酶（1,4β-内切葡聚糖酶，EC3.2.1：4）的最佳条件为30℃，5.5 pH。研究了桔梗MTCC 9620的生长动力学，显示出适合Monod模型以描述桔梗MTCC 9620在30°C孵育120 h的SSF中的生长模式。

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