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首页> 外文期刊>British Biotechnology Journal >Plant Regeneration via Somatic Embryogenesis in an Ethnomedicinal Plant Senna alata (L.) Roxb.
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Plant Regeneration via Somatic Embryogenesis in an Ethnomedicinal Plant Senna alata (L.) Roxb.

机译：在民族植物Senna alata（L.）Roxb中通过体细胞胚发生进行植物再生。

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Aim: To study the effect of various plant growth regulators (PGRs) for induction of somatic embryogenesis and plantlet regeneration from cotyledon and leaflet explants in Senna alata (L.) Roxb. (Syn. Cassia alata ) an important ethnomedicinal plant used in the treatment of fungal skin infections. Place and Duration of Study: Department of Biotechnology, Kakatiya University, Warangal, Telangana, India, between June 2013 to September 2014. Methodology: Cotyledon and leaflet segments (1-3 cm, 20 day old) were cultured on MS medium supplemented with 0.5 mg/L N6-Benzylaminopurine (BAP) /Thidiazuron (TDZ) in combination with various concentrations of plant growth regulators (PGRs) viz., Indole acetic acid (IAA)/ α-Naphthaleneacetic acid (NAA)/2,4-Dichlorophenoxy acetic acid (2,4-D). The percentage of somatic embryo induction, maturation and plantlet formation is calculated. Results: Maximum percentage of somatic embryogenesis (91%) was observed in cotyledon explants on MS medium augmented with 0.5 mg/L TDZ in combination with 3.0 mg/L NAA while the highest number of somatic embryos per explant (66.9±0.10) was formed in leaflet explants on MS medium supplemented with 0.5 mg/L BAP+3.0 mg/LNAA followed by 0.5 mg/L TDZ +3.0 mg/LNAA. Conclusion: Among the explants tested, cotyledon explants were proved to be efficient for induction of somatic embryogenesis and plantlet development compared to leaflet explants and MS medium fortified with TDZ is more effective compared to BAP in both the cotyledon as well as leaf explants. The developed plantlets were acclimatized and transferred to the research field. The regenerated plants were found to be similar to the donor plant phenotypically.
机译：目的：研究各种植物生长调节剂（PGR）诱导番泻叶（Roxb）子叶和小叶外植体的体细胞胚发生和小植株再生的作用。 （Syn。Cassia alata）是一种重要的民族药用植物，用于治疗真菌性皮肤感染。研究的地点和时间：2013年6月至2014年9月，在印度特伦甘纳邦的瓦兰加尔，卡卡蒂亚大学生物技术系。方法：子叶和小叶段（1-3厘米，20天大）在添加了0.5的MS培养基上培养mg / L N6-苄基氨基嘌呤（BAP）/噻二唑隆（TDZ）与各种浓度的植物生长调节剂（PGR）组合，即吲哚乙酸（IAA）/α-萘乙酸（NAA）/ 2,4-二氯苯氧基乙酸酸（2,4-D）。计算体细胞胚诱导，成熟和植株形成的百分比。结果：在0.5 mg / L TDZ和3.0 mg / L NAA组合的MS培养基上，子叶外植体中体细胞胚发生的最大百分比（91％），而每个外植体中形成的体细胞胚数量最高（66.9±0.10）在补充有0.5 mg / L BAP + 3.0 mg / LNAA，然后添加0.5 mg / L TDZ +3.0 mg / LNAA的MS培养基上的小叶外植体中进行检测。结论：在测试的外植体中，与子叶外植体相比，子叶外植体被证明能有效诱导体细胞胚发生和植株发育，并且在子叶和叶外植体中，以TDZ强化的MS培养基比BAP更有效。使已发育的幼苗适应环境并转移到研究领域。发现再生植物在表型上与供体植物相似。

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