• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biocontrol Science >Sterilization Effect of Wet Oxygen Plasma in the Bubbling Method
【24h】

Sterilization Effect of Wet Oxygen Plasma in the Bubbling Method

机译：鼓泡法中湿氧等离子体的灭菌作用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A new low-temperature sterilization method to replace the ethylene oxide gas sterilization is needed. Strong bactericidal effects of OH and O_(2)H radicals are well known. The purpose of this study was to evaluate the sterilization effect of wet oxygen ("O_(2)+H_(2)O") plasma in the bubbling method, confirming the effect of humidity. Sterility assurance was confirmed by using a biological indicator (Geobacillus stearothermophilus ATCC7953, Namsa, USA). One hundred and eight samples (10~(5) spores/carrier) were divided into three groups of 36 in each for treatment with a different type of gas (O_(2), O_(2)+H_(2)O, Air+H_(2)O). Plasma processing was conducted using a plasma ashing apparatus (13.56 MHz, PACK-3~(?), Y. A. C., Japan) under various gas pressures (13, 25, 50 Pa) and gas flows (50, 100, 200 sccm). Fixed plasma treatment parameters were power at 150 W, temperature of 60℃, treatment time of 10 min. The samples after treatment were incubated in trypticase soy broth at 58℃ for 72 h. The negative culture rate in the "O_(2)+H_(2)O" group was significantly (Mantel-Haenszel procedure, p<0.001) higher than in the other gas groups. It is suggested that the significant sterilization effect of the "O_(2)+H_(2)O" group depends on the bubbling method which is the method of introducing vapor into the chamber. The bubbling method seems able to generate OH and O_(2)H radicals in a stable way.
机译：需要一种新的低温灭菌方法来代替环氧乙烷气体灭菌。众所周知，OH和O_（2）H自由基具有很强的杀菌作用。这项研究的目的是评估鼓泡法中湿氧（“ O_（2）+ H_（2）O”）等离子体的灭菌效果，从而确认湿度的影响。通过使用生物指示剂（硬脂酸热地芽孢杆菌ATCC7953，美国南萨）来确认无菌性。将一百零八个样品（10〜（5）个孢子/载体）分成三​​组，每组36个，分别用不同类型的气体（O_（2），O_（2）+ H_（2）O，空气）处理+ H_（2）O）。使用等离子体灰化设备（13.56MHz，PACK-3（日本），Y.A.C，日本）在各种气压（13、25、50Pa）和气流（50、100、200sccm）下进行等离子体处理。固定的等离子处理参数为150 W的功率，60℃的温度，10分钟的处理时间。处理后的样品在胰蛋白酶大豆肉汤中于58℃孵育72小时。 “ O_（2）+ H_（2）O”组的阴性培养率显着高于（Mantel-Haenszel程序，p <0.001），高于其他气体组。建议“ O_（2）+ H_（2）O”基团的显着灭菌效果取决于鼓泡方法，该方法是将蒸气引入腔室的方法。鼓泡方法似乎能够以稳定的方式产生OH和O_（2）H自由基。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号