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首页> 外文期刊>Biocontrol Science >Role of the Histone-Like Nucleoid Structuring Protein (H-NS) in the Regulation of Virulence Factor Expression and Stress Response in Vibrio vulnificus
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Role of the Histone-Like Nucleoid Structuring Protein (H-NS) in the Regulation of Virulence Factor Expression and Stress Response in Vibrio vulnificus

机译：类组蛋白核样结构蛋白（H-NS）在调节 外伤弧菌 的毒力因子表达和应激反应中的作用

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摘要

Temperature is one of the important parameters regulating the expression of virulence factors in bacteria. The global regulator, a histone-like nucleoid structuring protein (H-NS), is known to play a crucial role in this regulation. In the present study, we first clarified the role of H-NS in the temperature-dependent regulation of virulence factor production in Vibrio vulnificus , including that of the cytolytic toxin (V. vulnificus hemolysin: VVH) and the proteolytic enzyme (V. vulnificus protease: VVP). The expression of hns itself was subjected to temperature regulation, where hns was expressed more at 26℃ than at 37℃. VVH production and the expression of its gene vvhA were increased by disruption of the hns gene. H-NS appeared to affect the vvhA expression by the well-documented transcriptional silencing mechanism. On the other hand, hns disruption resulted in the reduction of VVP production and the expression of its gene vvpE . H-NS was suggested to positively regulate vvpE expression through the increase in the level of the rpoS mRNA. Moreover, H-NS was found to contribute to the survival of V. vulnificus in stressful environments. When compared to the wild type strain, the hns mutant exhibited reduced survival rates when subjected to acidic pH, hyperosmotic and oxidative stress.
机译：温度是调节细菌中毒力因子表达的重要参数之一。全局调节剂，一种组蛋白样核糖结构蛋白（H-NS），在该调节中起着至关重要的作用。在本研究中，我们首先阐明了H-NS在创伤弧菌毒力因子产生的温度依赖性调节中的作用，包括溶细胞毒素（v。vulnificus溶血素：VVH）和蛋白水解酶（V. vulnificus蛋白酶：VVP）。 hns本身的表达受到温度调节，其中hns在26℃比37℃表达更多。通过破坏hns基因，增加了VVH的产生及其基因vvhA的表达。 H-NS似乎通过充分证明的转录沉默机制影响vvhA表达。另一方面，hns破坏导致VVP产生减少及其基因 vpE表达。有人建议H-NS通过增加rpoS mRNA的含量来正向调节vvpE的表达。此外，发现H-NS有助于V的存活。压力环境下的创伤。当与野生型菌株比较时，hns突变体在经受酸性pH，高渗和氧化应激时表现出降低的存活率。

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