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首页> 外文期刊>BMC Microbiology >Expression and subcellular localization of kinetoplast-associated proteins in the different developmental stages of Trypanosoma cruzi
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Expression and subcellular localization of kinetoplast-associated proteins in the different developmental stages of Trypanosoma cruzi

机译：克鲁氏锥虫不同发育阶段动塑料相关蛋白的表达和亚细胞定位

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Background The kinetoplast DNA (kDNA) of trypanosomatids consists of an unusual arrangement of circular molecules catenated into a single network. The diameter of the isolated kDNA network is similar to that of the entire cell. However, within the kinetoplast matrix, the kDNA is highly condensed. Studies in Crithidia fasciculata showed that kinetoplast-associated proteins (KAPs) are capable of condensing the kDNA network. However, little is known about the KAPs of Trypanosoma cruzi, a parasitic protozoon that shows distinct patterns of kDNA condensation during their complex morphogenetic development. In epimastigotes and amastigotes (replicating forms) the kDNA fibers are tightly packed into a disk-shaped kinetoplast, whereas trypomastigotes (non-replicating) present a more relaxed kDNA organization contained within a rounded structure. It is still unclear how the compact kinetoplast disk of epimastigotes is converted into a globular structure in the infective trypomastigotes. Results In this work, we have analyzed KAP coding genes in trypanosomatid genomes and cloned and expressed two kinetoplast-associated proteins in T. cruzi: TcKAP4 and TcKAP6. Such small basic proteins are expressed in all developmental stages of the parasite, although present a differential distribution within the kinetoplasts of epimastigote, amastigote and trypomastigote forms. Conclusion Several features of TcKAPs, such as their small size, basic nature and similarity with KAPs of C. fasciculata, are consistent with a role in DNA charge neutralization and condensation. Additionally, the differential distribution of KAPs in the kinetoplasts of distinct developmental stages of the parasite, indicate that the kDNA rearrangement that takes place during the T. cruzi differentiation process is accompanied by TcKAPs redistribution.
机译：背景锥虫的运动塑料DNA（kDNA）由连接到单个网络的环状分子的不寻常排列组成。分离的kDNA网络的直径类似于整个细胞的直径。但是，在动质体基质中，kDNA高度浓缩。 Fasciculata的研究表明，动塑料相关蛋白（KAP）能够浓缩kDNA网络。然而，关于锥虫锥虫的KAP知之甚少，它是一种寄生的原生动物，在其复杂的形态发生过程中显示出独特的kDNA缩合模式。在附生鞭毛体和无鞭毛体（复制体）中，kDNA纤维紧密堆积在圆盘形动植物体中，而锥po（非复制体）呈现出更宽松的kDNA组织，包含在圆形结构中。尚不清楚表鞭毛体的致密运动体圆盘如何在感染性鞭毛体中转化为球形结构。结果在这项工作中，我们分析了锥虫基因组中的KAP编码基因，并克隆并在克鲁维酵母中表达了两个与动塑料相关的蛋白：TcKAP4和TcKAP6。这种小的碱性蛋白在寄生虫的所有发育阶段均表达，尽管在表鞭毛，鞭毛体和锥鞭毛体形式的运动塑料中存在差异分布。结论TcKAPs的小尺寸，基本性质和与棒状线虫KAP的相似性等一些特征与DNA电荷中和和凝结的作用一致。此外，KAPs在寄生虫不同发育阶段的运动塑料中的差异分布表明，在克鲁维氏酵母分化过程中发生的kDNA重排伴随着TcKAPs重新分布。

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