• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biocontrol Science >LC-MS Analysis of the Emetic Toxin, Cereulide, Produced by Bacillus cereus
【24h】

LC-MS Analysis of the Emetic Toxin, Cereulide, Produced by Bacillus cereus

机译：蜡样芽孢杆菌产生的催吐毒素，蜡样内酯的LC-MS分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A quantitative and chemical assay of cereulide produced in the cultures by some strains of Bacillus cereus was performed on a HPLC and a ESI electrospray ion trap mass analyzer, using the synthetic cereulide as a standard. All 20 strains of emetic B. cereus were found to produce 27 - 740 ng/ml of cereulide by the LC-MS analysis. In contrast, none of the 10 diarrheal strains produced it. 10~(2) cfu/ml of the cereulide producible strain with a 210 ng/ml yield was inoculated into the 10% suspensions of 14 food products, and was incubated at 32°C for 24h. The B. cereus counts in the cultures grew in the order of 10~(8) to 10~(9) cfu/ml, although the bacteria could not grow in fruits, and the yields of cereulide ranged from 5.18μg in curry to 0.03μg/g of raw material and/or powder material, except for fruits. These culture supernatants were also tested for the biological activity in the HEp-2 cell culture assay. Consequently, a certain correlation was shown between the yields of cereulide and the HEp-2 vacuolation activities. In addition, the supernatants were administered i.p. to 5 Suncus marinus test animals. The emetic dose was calculated to be approximately 16μg/kg.
机译：使用合成的蜡样内酯作为标准品，在HPLC和ESI电喷雾离子阱质量分析仪上对某些蜡样芽胞杆菌菌株在培养物中产生的蜡样内酯进行了定量和化学分析。催吐的所有20株B。通过LC-MS分析发现蜡状杆菌产生27-740 ng / ml的蜡状内酯。相反，十种腹泻菌株均未产生。在14种食品的10％悬浮液中接种10〜（2）cfu / ml的蜡状内酯可生产菌株，产量为210 ng / ml，并在32°C下孵育24h。 B。尽管该细菌不能在果实中生长，但培养物中的蜡状菌计数却以10〜（8）cfu / ml的量级增长，从10.（8）cfu / ml升至0.03μg/ g。原料和/或粉末材料，水果除外。还在HEp-2细胞培养测定中测试了这些培养上清液的生物学活性。因此，证明了蜡样内酯的产率与HEp-2空泡化活性之间存在一定的相关性。另外，对上清液进行腹膜内注射。到5只Suncus marinus测试动物。催吐剂量经计算约为16μg/ kg。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号