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首页> 外文期刊>British Biotechnology Journal >Cell Proliferation Using Broccoli Leaf Cuttingin vitro Culture: Its Biochemical and AntioxidantProperties
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Cell Proliferation Using Broccoli Leaf Cuttingin vitro Culture: Its Biochemical and AntioxidantProperties

机译：西兰花切叶在体外培养中的细胞增殖：其生化和抗氧化性能

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Significance of the study: Tissue or cell culture keeps a significant role in micro-propagation in the plant production industry. Nowadays it has been successfully performed from many species in plant. Millions of explants can be produced by tissue or cell culture per year in any plant production industry.Aim: The study was conducted to investigate the root, shoot and leaf formation from the leaf cutting in vitro culture of broccoli (Brassica oleracea var italic).Methodology: Different BAP (6-Benzylaminopurine) and IBA (Indole-butaric-acid) hormone (0.25, 0.50, 1.0, 1.50, 2.0 2.5, 3.0 and 3.5 mg/l) concentrations in combination with MS media was used to culture leaf cutting in vitro.Results: The results showed that there was no shoot formed, but a few leaves and bulky callus proliferation were appeared. The highest number (6.75) of root proliferation was found in the concentration of 2.0 mg/l IBA + 0.25 mg/l BAP combination. 2,2-diphenyl-2-picrylhydarzyl (DPPH) free radical scavenging potential was higher (70%) in leaves extract than in callus extracts (46%) at concentration of 10 mg/ml.Conclusion: The present results conclude that it is better to use the combination of BAP and IBA at different concentration to produce root proliferation, leaf initiation and callus formation in broccoli.
机译：研究的意义：组织或细胞培养在植物生产行业的微繁殖中保持着重要作用。如今，已成功地从植物中的许多种中进行了分离。在任何植物生产行业中，每年通过组织或细胞培养可产生数百万个外植体。目的：进行这项研究以调查西兰花（Brassica oleracea var italic）的切叶体外培养中的根，芽和叶的形成。方法：将不同浓度的BAP（6-苄基氨基嘌呤）和IBA（吲哚-丁酸）激素（0.25、0.50、1.0、1.50、2.0、2.5、3.0和3.5 mg / l）与MS培养基一起用于培养切叶结果：结果表明，没有芽形成，但是出现了一些叶子和大的愈伤组织增殖。在2.0 mg / l IBA + 0.25 mg / l BAP组合的浓度下，根增殖的最高数量（6.75）被发现。在浓度为10 mg / ml的情况下，叶片提取物中的2,2-二苯基-2-picylhydarzyl（DPPH）清除自由基的能力（46％）高于叶片提取物中的（70 ％）。结论：本研究结果得出结论：最好使用不同浓度的BAP和IBA的组合在西兰花中产生根增殖，叶片萌生和愈伤组织形成。

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