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首页> 外文期刊>BMC Microbiology >In vivo involvement of polymorphonuclear neutrophils in Leishmania infantum infection
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In vivo involvement of polymorphonuclear neutrophils in Leishmania infantum infection

机译：多形核中性粒细胞在体内参与婴儿利什曼原虫感染

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The role of lymphocytes in the specific defence against L. infantum has been well established, but the part played by polynuclear neutrophil (PN) cells in controlling visceral leishmaniasis was much less studied. In this report we examine in vivo the participation of PN in early and late phases of infection by L. infantum. Promastigote phagocytosis and killing occurs very early after infection, as demonstrated by electron microscopy analyses which show in BALB/c mouse spleen, but not in liver, numerous PN harbouring ultrastructurally degraded parasites. It is shown, using mAb RB6-8C5 directed against mature mouse granulocytes, that in chronically infected mice, long-term PN depletion did not enhance parasite counts neither in liver nor in spleen, indicating that these cells are not involved in the late phase of L. infantum infection. In acute stage of infection, in mouse liver, where L. infantum load is initially larger than that in spleen but resolves spontaneously, there was no significant effect of neutrophils depletion. By contrast, early in infection the neutrophil cells crucially contributed to parasite killing in spleen, since PN depletion, performed before and up to 7 days after the parasite inoculation, resulted in a ten-fold increase of parasite burden. Taken together these data show that neutrophil cells contribute to the early control of the parasite growth in spleen but not in liver and that these cells have no significant effect late in infection in either of these target organs.
机译：淋巴细胞在针对婴儿乳杆菌的特异性防御​​中的作用已被很好地确立，但对多核中性粒细胞（PN）细胞在控制内脏利什曼病中所起的作用却鲜有研究。在本报告中，我们研究了PN在婴儿乳杆菌感染的早期和晚期的参与情况。电子显微镜分析表明，在BALB / c小鼠脾脏中，但在肝脏中，无数PN携带超微结构降解的寄生虫，前鞭毛吞噬作用和杀伤在感染后很早就发生。使用针对成熟小鼠粒细胞的mAb RB6-8C5显示，在慢性感染的小鼠中，长期PN耗竭并没有增加肝脏和脾脏中的寄生虫计数，这表明这些细胞不参与肝癌的晚期。婴儿乳杆菌感染。在感染的急性阶段，在小鼠肝脏中，婴儿乳杆菌的负荷最初大于脾脏中的负荷，但自发消退，对中性粒细胞耗竭没有明显影响。相比之下，在感染早期，嗜中性粒细胞在脾脏中对寄生虫的杀伤至关重要，因为在寄生虫接种之前和之后7天进行的PN耗竭导致寄生虫负担增加了十倍。这些数据加在一起表明，嗜中性粒细胞有助于脾脏中而不是肝脏中寄生虫生长的早期控制，并且这些细胞在这些靶器官中的任何一个的感染后期都没有明显的作用。

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