首页> 外文期刊>British Biotechnology Journal >Genetic Variations in Asparagus racemosus, anEndangered Medicinal Herb Endemic to India UsingRAPD Markers
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Genetic Variations in Asparagus racemosus, anEndangered Medicinal Herb Endemic to India UsingRAPD Markers

机译:使用RAPD标记对印度特有的濒危药用草药芦笋种的遗传变异

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Aim: To study the genetic diversity in Asparagus racemosus germplasm using RAPD molecular markers for its better conservation and utilization.Study Design: RAPD markers used to check genetic diversity in Asparagus racemosus using different softwares.Place and Duration of Study: Department of Bio & Nano Technology, Guru Jambheshwar University of Science & Technology, Hisar-125001 between May 2013 to June 2014.Methodology: A total of 60 RAPD markers used to check polymorphism at genetic level among 60 asparagus genotypes. PCR amplified bands were scored as 0 and 1 for absence and presence. The binary data so obtained used to reveal genetic polymorphism via NTSYS, POPGENE and AMOVA analysis.Results: A significant level of genetic diversity (81.48%) among all genotypes was assessed by using RAPD molecular markers. Out of 60, 49 RAPD primers produced 425 polymorphic loci. The value of Jaccard’s coefficient varied from 0.48 to 0.97 for RAPD. OPB-15 primer proved to be the most polymorphic marker among all used. The POPGENE analysis revealed 44.44, 79.01 and 64.20% polymorphism for RAPD analysis in groups with low, intermediate and high saponin content. The overall value of Shannon’s index and Nei’s genetic diversity was 0.3402 & 0.2169 for RAPD marker system.Conclusion: These results showed RAPD marker system useful in detecting significant genetic polymorphism among genotypes which can be used for production and conservation of improved genotypes.
机译:目的:利用RAPD分子标记研究芦笋种质的遗传多样性,以更好地保存和利用研究设计:使用不同软件通过RAPD标记检查芦笋的遗传多样性研究地点和持续时间:生物与纳米系2013年5月至2014年6月,古鲁贾姆布什瓦尔科技大学科技学院,Hisar-125001。方法:共有60种RAPD标记用于在60种芦笋基因型中从基因水平检查多态性。 PCR扩增条带的不存在和存在分别为0和1。这样获得的二元数据用于通过NTSYS,POPGENE和AMOVA分析揭示遗传多态性。结果:使用RAPD分子标记评估了所有基因型中显着水平的遗传多样性(81.48%)。在60种RAPD引物中,有49种产生了425个多态位点。对于RAPD,Jaccard系数的值从0.48到0.97不等。 OPB-15引物被证明是所有使用中最多态的标记。 POPGENE分析显示低,中和高皂苷含量的组中RAPD分析的多态性为44.44%,79.01%和64.20%。对于RAPD标记系统,Shannon指数和Nei遗传多样性的总价值分别为0.3402和0.2169。结论:这些结果表明,RAPD标记系统可用于检测基因型之间的重要遗传多态性,可用于生产和保存改良的基因型。

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