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Biotechnological Application of Cassava-DegradingFungal (CDF) Amylase in Broiler Feed Formulation

机译:木薯降解真菌(CDF)淀粉酶在肉鸡饲料配方中的生物技术应用

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In this study, an attempt was made to substitute maize (Zea mays L.) content of broiler starter feed with ground cassava (Manihot esculenta Crantz) peels enzymatically improved with amylase-producing fungi with a view to having a cost-effective yet nutritious and health-friendly feed. The biochemical components of the formulated feeds were determined as well as the effect of the feeds on some biochemical parameters in the broiler chicks. Six starter feeds tagged Control Feed, 20%CPFG, 40%CPFG, 60%CPFG, 80%CPFG and 100%CPFG were formulated with respect to variations in maize and cassava peel contents. The results showed a significant (p<0.05) increase in total polysaccharide contents of Feeds 20%CPFG, 60%CPFG, 80%CPFG and 100%CPFG compared to the Control Feed (191.4±14.5 mg/g of feed). Total soluble protein and reducing sugar contents were statistically different in most of the feeds (p<0.05). All the feeds contain relatively high amounts of total phenol (>70 mgCE/g of feed) and most of them comparatively high in anthocyanin relative to the control feed anthocyanin content (225.4±12.2 mg/g of feed). The highest weight gain (108.0±3.0 g) was observed in broiler chicks fed the feed ration containing 60% cassava peels improved with amylase-producing fungi and 40% maize (60%CPFG). Broiler chicks fed the formulated feeds, including the control, exhibited over 40% inhibition against 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical. The serum activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in birds fed the compounded feeds and control slightly differed except in group 60%CPFG. It was concluded that the maize content in broiler feed can be replaced with cassava peels improved with fungal amylase upto a maximum of 60%. This would significantly decrease the overall cost of broiler feed production without compromising the nutritional, antioxidant and health-friendly potentials of the feed.
机译:在这项研究中,尝试用生产淀粉酶的真菌用酶促改良的木薯(Manihot esculenta Crantz)果皮代替肉鸡起初饲料中的玉米(Zea mays L.)含量,以期获得具有成本效益,营养丰富且营养丰富的产品。健康的饲料。确定了配制饲料的生化成分,以及饲料对肉仔鸡某些生化参数的影响。根据玉米和木薯果皮含量的变化,配制了六种标记为对照饲料的起始饲料,20%CPFG,40%CPFG,60%CPFG,80%CPFG和100%CPFG。结果表明,与对照饲料(191.4±14.5 mg / g饲料)相比,饲料20 %CPFG,60 %CPFG,80 %CPFG和100 %CPFG的总多糖含量显着增加(p <0.05) )。在大多数饲料中,总可溶性蛋白质和还原糖含量在统计学上存在差异(p <0.05)。所有进料中都含有相对较高的总酚含量(> 70 mgCE / g进料),并且相对于对照进料中花青素含量(225.4±12.2 mg / g进料),其中大多数花色苷的含量相对较高。在饲喂含60%木薯皮的饲粮的肉鸡中观察到最高增重(108.0±3.0 g),其中木薯果皮的淀粉酶产生真菌和40 %%的玉米(60%CPFG)改善了。饲喂包括对照在内的配方饲料的肉鸡对2,2-二苯基-1-吡啶并肼基(DPPH)自由基显示出40%以上的抑制作用。饲喂复合饲料和对照的家禽的丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)的血清活性略有不同,除了60%CPFG组。结论是,用真菌淀粉酶提高到最高60%的木薯皮可以代替肉鸡饲料中的玉米含量。这将显着降低肉鸡饲料生产的总成本,而不会损害饲料的营养,抗氧化剂和对健康有益的潜力。

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