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首页> 外文期刊>British Biotechnology Journal >DNA Sequence Characteristics and Phylogenetics ofThree Oligonucleotides Markers on Clariid Species
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DNA Sequence Characteristics and Phylogenetics ofThree Oligonucleotides Markers on Clariid Species

机译：梭织物种上三个寡核苷酸标记的DNA序列特征和系统发育

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Aims: The aim of this study is to express the profiles of three oligonucleotide markers corresponded to reproductive genes that may be different between the two Clariid species (Clarias gariepinus and Heterobranchus bidorsalis) and their phylogenetics.Methodology: Total DNA isolation was carried out on the whole blood of the two strains of Clariid species – 100 species Clarias gariepinus male and female (1.2 – 1.5 kg, 34 – 52 cm) separately; 100 species Heterobranchus bidorsalis (1.7 – 2.2 kg, 38 – 60 cm) respectively using the Quick-gDNA Zymo research kit. Having ascertained the DNA stability on 0.8% agarose gel, NCBI database and Clustal analyses were employed to design primers to reproductive genes that may be different between the two catfishes and may participate in their differential reproducibility. We have used quantitative real-time PCR to investigate the expression of three selected oligonucleotides markers on the catfish. CLC Sequence viewer 7 software was used to analyze the nucleotide alignment percentage and develop the phylogenetics tree.Place and Duration of Study: The study was carried out in the Biotechnology Centre, Federal University of Agriculture, Abeokuta Nigeria between January and July 2014.Results: We observed a dimorphic expression pattern of the three marker genes in relation to strains and sex differentiation, indicating that sox9a retained its function in testis, Figα was highly expressed in the female and Cyp19a1b was up-regulated in male C. gariepinus than male H. bidorsalis catfish species. The phylogenetic tree showed that male Heterobranchus and female Clarias were closer irrespective of male or female while male Clarias differed from the two.Conclusion: To date, these three genes, Sox 9a, Figα and Cyp19a1b have been detected in many fishes, but little or no data has been reported in African catfishes. The findings from this study might be used as the target gene for catfish gender regulation.
机译：目的：这项研究的目的是表达与两个Clariid物种（Clarias gariepinus和Heterobranchus bidorsalis）的生殖基因相对应的三个寡核苷酸标记的概况及其系统发育方法。这两种科莱氏菌属的全血–分别为100种Claris gariepinus雄性和雌性（1.2 – 1.5 kg，34 – 52 cm）;使用Quick-gDNA Zymo研究试剂盒分别得到100种Heterobranchus bidorsalis（1.7 – 2.2 kg，38 – 60 cm）。在0.8％的琼脂糖凝胶上确定了DNA稳定性后，使用NCBI数据库和Clustal分析设计了两个to鱼之间可能不同并可能参与其差异重现性的生殖基因引物。我们已经使用定量实时PCR来研究three鱼上三个选定的寡核苷酸标记的表达。研究的地点和持续时间：研究于2014年1月至7月在尼日利亚阿贝库塔的联邦农业大学生物技术中心进行。我们观察到了三个标记基因与菌株和性别分化有关的双态表达模式，表明sox9a在睾丸中保留了其功能，在雌性中图α高表达，而雄性C.gariepinus中的Cyp19a1b高于雄性H.阔叶cat鱼种。系统进化树表明，不论雌雄，杂种雄性和雌性鞭毛均较近，而雄性鞭毛与两者的异种。结论：迄今为止，在许多鱼类中都检测到了这三个基因Sox 9a，Figα和Cyp19a1b，但很少或没有关于非洲鱼的报道。这项研究的发现可以用作cat鱼性别调控的目标基因。

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