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Inter Simple Sequence Repeats Polymorphismin Sudanese Sorghum bicolor (L.) MoenchAccessions

机译:间简单序列在苏丹高粱双色(L.)MoenchAccessions中重复多态性

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Aims: Investigate the degree of polymorphism using 41 ISSR (Inter-simple sequence repeat) markers in 50 sorghum accessions from 11 different regions in Sudan and Republic of South Sudan.Study Design: UPGMA cluster analysis using STATISTCA- SPSS software Ver. 9.Place and Duration of Study: Department of Molecular Biology, Commission for Biotechnology and Genetic Engineering, National Center for Research, Khartoum, Sudan (2010-2012).Methodology: 50 sorghum accessions with important agronomic traits, representing 11 regions in Sudan and Republic of South Sudan were assayed for polymorphism using Inter-simple sequence repeats (ISSRs). Seven primers out of 41 tested (807, 808, 810, 814, 848, 872 and 879) showed high polymorphism among the Sorghum accessions. Results: The results indicated 75 polymorphic bands out of 78 bands with percentage of polymorphic bands of 97%. UPGMA analysis showed ISSR distance matrix ranged between (0.04-0.47) which reflected high genetic diversity. The ISSR UPGMA dendrogram showed high molecular variance within regions. Based on the results of this study ISSR technique showed differences among closely related accessions of sorghum. Also it proved to be useful technique to study genetic variation among the Sudanese Sorghum accessions.Conclusion: Sorghum accessions from Sudan exhibits high genetic variation within and among regions. ISSR marker technique used in this study proved that it is efficient and could be very useful for breeders and researchers community in various fields of sorghum improvement in Sudan.
机译:目的:研究苏丹和南苏丹共和国11个不同地区的50个高粱品种中的41个ISSR(简单序列间重复)标记的多态性。研究设计:使用STATISTCA-SPSS软件Ver.1进行UPGMA聚类分析。 9,研究地点和持续时间:苏丹喀土穆国家研究中心生物技术和遗传工程委员会分子生物学系(2010-2012年)方法:50种具有重要农艺性状的高粱品种,分别代表苏丹和苏丹的11个地区使用简单序列间重复序列(ISSR)分析南苏丹共和国的多态性。在41个测试的引物(807、808、810、814、848、872和879)中有7个引物在高粱种之间表现出高多态性。结果:结果表明78个条带中的75个多态条带,多态条带百分比为97%。 UPGMA分析表明,ISSR距离矩阵介于(0.04-0.47)之间,这反映了高遗传多样性。 ISSR UPGMA树状图显示区域内的分子差异很大。根据这项研究的结果,ISSR技术显示了高粱紧密相关种质之间的差异。结论:苏丹高粱种在区域内和区域间遗传变异高。这项研究中使用的ISSR标记技术证明,它是有效的,并且对于苏丹高粱改良各个领域的育种者和研究人员社区可能非常有用。

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