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Assessment of Toxic Metals in Dairy Milk andAnimal Feed in Peshawar, Pakistan

机译:巴基斯坦白沙瓦牛奶和动物饲料中有毒金属的评估

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Aims: To assess the concentration of toxic heavy metals [Cadmium, Copper, Lead and Nickle] in dairy milk and animal feed of urban and rural areas and correlate them.Study Design: It was a cross-sectional comparative study.Place and Duration of Study: The study was conducted on the dairy milk and animal feed in milk farms of Peshawar. The samples were collected during June – July of 2010.Methodology: The study area was divided into two zones: urban area which was located in the centre of the city and rural area comprising of peri urban villages. Fifty milk samples were collected, 30 from urban and 20 from rural areas. Feed samples were also collected. Milk and feed samples were prepared by wet digestion method using HNO3 and H2O2 as described by Association of Official Analytical Chemists (AOAC) of North America, 1990. This was followed by analysis on Atomic Absorption Spectrometer. Results: The results showed that the mean concentration of toxic heavy metals under study namely Cadmium, Copper, Lead and Nickle were greater in dairy milk than the maximum residue limits (MRLs) of these elements as given by Joint Expert Committee on Food and Agriculture and World Health Organization. In comparison of urban and rural areas it was found that higher concentration of Cadmium, Copper, and Nickle was found in dairy milk samples from urban areas than rural areas but there was no significant difference in Lead concentration of the two areas. Their concentration in urban and rural samples were respectively Cadmium 0.69 and 0.037 with P-value <0.001, Copper 1.40 and 0.09 with P-value 0.001, Nickel 0.806 and 0.024 with P-value <0.001, Lead 2.243 and 2.082 with P -value 0.026. However in this study all these elements were within the permissible limits in animal feed except for copper in cotton khal which was 83.3mg kg-1 against the MRL of 10 mg kg-1 given by National Research Council, NRC, 2005. Also there was no significant difference in the concentration of these elements in feed of urban and rural areas. Correlation study between the concentration of these elements in milk and feed showed positive pearson’s correlation coefficient between the two particularly for copper (0.451) in sugarcane khal and (0.341) in wheat grain, Nickle (0.342) in maize, and (0.30) in wheat bran and cotton khal. Conclusion: The concentrations of toxic metals were higher than the maximum residue limits in milk but were within their normal limits in animal feed suggesting that milk is being contaminated by various other sources but not farm feed. Comparison between urban and rural areas showed higher concentration in urban milk samples as compared to rural except for lead probably because urban farms are located in polluted areas in the centre of the city. However no significant difference was found in animal feed of urban and rural areas because it is supplied from common source.
机译:目的:评估城市和农村地区的牛奶和动物饲料中有毒重金属[镉,铜,铅和镍]的浓度并将它们相关联。研究设计:这是一项横断面比较研究。研究:该研究是在白沙瓦牛奶农场的奶和动物饲料上进行的。样本于2010年6月至7月收集。方法:研究区域分为两个区域:位于城市中心的市区和由周边乡村组成的农村地区。收集了50份牛奶样品,其中30份来自城市,20份来自农村。还收集了饲料样品。按照北美官方分析化学家协会(AOAC)的描述,使用HNO3和H2O2通过湿消化法制备牛奶和饲料样品。随后在原子吸收光谱仪上进行分析。结果:结果表明,所研究的牛奶中有毒重金属的平均浓度(镉,铜,铅和镍)高于食品和农业联合专家委员会给出的这些元素的最大残留限量(MRL)。世界卫生组织。通过比较城市和农村地区,发现城市地区的牛奶样本中镉,铜和镍的浓度高于农村地区,但两个地区的铅浓度没有显着差异。它们在城市和农村样品中的浓度分别为P值<0.001的镉0.69和0.037,P值0.001的铜1.40和0.09,P值<0.001的镍0.806和0.024,铅2.243和2.082以及P值0.026 。但是,在这项研究中,所有这些元素都在动物饲料的允许范围内,除了棉花卡哈尔中的铜含量为83.3mg kg-1,而美国国家研究委员会(National Research Council,NRC)给出的最大残留限量为10 mg kg-1。这些元素在城乡地区饲料中的浓度没有显着差异。牛奶和饲料中这些元素的浓度之间的相关性研究显示,两者之间的皮尔逊相关系数呈正相关,尤其是甘蔗哈尔铜(0.451)和小麦籽粒中的铜(0.341),玉米中镍(0.342)和小麦中(0.30)两者之间的正皮尔逊相关系数。麸皮和棉花喀尔。结论:有毒金属的浓度高于牛奶中的最大残留限量,但在动物饲料中仍在其正常限量之内,这表明牛奶正受到其他各种来源的污染,但不受农场饲料的污染。城乡之间的比较显示,除铅外,城市牛奶样品中的浓度要比农村高,这可能是因为城市农场位于城市中心的污染地区。然而,由于其来源是共同的,因此在城乡动物饲料中没有发现显着差异。

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