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首页> 外文期刊>British Biotechnology Journal >Sequence Homology Studies of PhospholipaseA2-like Gene from Bloodstream form ofTrypanosoma brucei
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Sequence Homology Studies of PhospholipaseA2-like Gene from Bloodstream form ofTrypanosoma brucei

机译：布氏锥虫血流形式的磷脂酶A2基因的序列同源性研究

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Aim: This work focused on the sequence homology studies of the enzyme, phospholipase A2 (PLA2), in Trypanosoma brucei obtained from the blood of bull in Federe, Plateau State, Nigeria, West AfricaPlace and Duration of Study: Department of Biochemistry, University of Jos, Nigeria; Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria, Department of Biotechnology, NVRI, Vom, Nigeria; between June 2009 and September 2011.Methodology: T. brucei grown in rats were harvested and separated using diethyl amino ethyl (DEAE) cellulose chromatography. From the parasites’ genomic DNA the PLA2-like gene was amplified using consensus primers. The amplicon was cloned unto pMal-2cE vector and confirmed using direct PCR and restriction enzyme analyses. The PLA2 gene and translated protein sequences were studied using National Center for Biotechnology Information (NCBI) Conserved Domain Search Tool and Conserved Domain Architectural Retrieval Tool Results: Analyses of the 1344bp gene sequence using bioinformatics tools showed that it is very closely related to PLA2 sequences of T. brucei (TREU 927) and T. b. gambiense. Motifs that are unique to PLA2 (FSHGL) and lipases (GHSFG) were found to be present in the query sequence. The domains present in the studied sequence agreed closely with those of the human platelet activating factor acetyl hydrolase (PAF-AH). There was also a good sequence resemblance with PLA2s from T. cruzi, Metarhizium amisop, Metarphizium acridu and PAF-AH in terms of architecture. Conclusion: The PLA2-like gene isolated from the blood stream form of Trypanosoma brucei and studied was found to posses the domains and motifs unique to PLA2s and lipases and so homology was established among the proteins.
机译：目的：这项工作侧重于从非洲费德雷，尼日利亚高原州，西非的公牛血中获得的布鲁氏锥虫中的酶磷脂酶A2（PLA2）的序列同源性研究地点和研究持续时间：墨西哥大学生物化学系尼日利亚乔斯;尼日利亚扎里亚，阿马杜贝洛大学生物化学系，尼日利亚沃姆，NVRI生物技术系;在2009年6月至2011年9月之间。方法：使用二乙氨基乙基（DEAE）纤维素色谱法收集大鼠分离的布鲁氏杆菌。使用共有引物从寄生虫的基因组DNA中扩增出PLA2样基因。将扩增子克隆到pMal-2cE载体中，并使用直接PCR和限制酶分析进行确认。使用国家生物技术信息中心（NCBI）保守域搜索工具和保守域结构检索工具对PLA2基因和翻译的蛋白质序列进行了研究。结果：使用生物信息学工具对1344bp基因序列进行的分析表明，该序列与PLA2序列非常相关。布氏锥虫（TREU 927）和T. b。甘比森。发现查询序列中存在PLA2（FSHGL）和脂肪酶（GHSFG）独有的基序。存在于研究序列中的结构域与人血小板活化因子乙酰水解酶（PAF-AH）的结构域非常一致。就构筑而言，与来自克鲁氏T，拟南芥，拟南芥和PAF-AH的PLA2也具有良好的序列相似性。结论：从布鲁氏锥虫的血流形式分离并研究了PLA2样基因，发现它具有PLA2和脂肪酶独特的结构域和基序，从而在蛋白质之间建立了同源性。

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