• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>British Biotechnology Journal >Influence of Type and Age of Primary SomaticEmbryo on Secondary and Cyclic SomaticEmbryogenesis of Cassava (Manihot esculentaCrantz)
【24h】

Influence of Type and Age of Primary SomaticEmbryo on Secondary and Cyclic SomaticEmbryogenesis of Cassava (Manihot esculentaCrantz)

机译：原代体细胞胚的类型和年龄对木薯继代和周期性体细胞胚发生的影响（Manihot esculentaCrantz）

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study investigated the influence of age of the cotyledons, cut from primary somatic embryos (PSE) developed from shoot meristems (SM) or immature leaf lobes (LL), on secondary somatic (SSE) and cyclic (CSE) embryogenesis of two cassava cultivars at the Central Biotech Laboratory, International Institute of Tropical Agriculture (IITA), Ibadan, between 2006 and 2010. A completely randomized design with three replicates was used for the study. Only PSE at the age of 4 weeks recorded significant (P<0.05) differences in SSE frequency and efficiency between the SM and LL sources. CSE production was highest using 0 to 4 weeks old SSE cotyledons, and significant (P<0.05) differences were only recorded between the SM and LL sources when the age of the SSE cotyledons was older than 6 weeks. The CSE frequencies from the SM source were significantly greater than that from the LL source when 8 and 10 week-old SSE cotyledons were used. The CSE frequencies from SM (81%, 82%) were still significantly higher than those from LL (41%, 40%) at the 5th and 6th cycles respectively while the CSE efficiency only differed at the 4th cycle, with SM (7.1) being significantly more than from LL (5.2). These results also enrich the literature by specifying the age of somatic cotyledon suitable for further somatic embryogenic cycles; 0 to 8 weeks for SSE and 0 to 5 weeks for CSE, irrespective of the cultivar (TME 12 or Kibaha) or source (SM or LL). This study further concluded that cyclic embryos should be discarded after the 4th CSE cycle and fresh starting material should be used to restart the somatic embryogenic process.
机译：这项研究调查了从分生组织（SM）或未成熟叶瓣（LL）发育的初级体细胞胚（PSE）切下的子叶的年龄对两个木薯品种的次级体细胞（SSE）和循环（CSE）胚胎发生的影响在2006年至2010年之间，在伊巴丹国际热带农业研究所（IITA）的中央生物技术实验室中进行了研究。该研究使用了完全随机的设计，包括三个重复样本。只有4周龄的PSE在SM和LL来源之间记录SSE频率和效率的显着（P <0.05）差异。使用0至4周龄的SSE子叶，CSE产量最高，只有当SSE子叶的年龄大于6周时，才在SM和LL来源之间记录显着（P <0.05）差异。当使用8和10周龄的SSE子叶时，SM来源的CSE频率显着高于LL来源。 SM的CSE频率（81 ％，82 ％）仍然分别显着高于LL的CSE频率（41 ％，40 ％）在第5和第6周期，而CSE效率仅在第4周期有所不同。 SM（7.1）明显高于LL（5.2）。通过指定适合进一步体细胞胚发生周期的体细胞子叶的年龄，这些结果也丰富了文献。 SSE为0到8周，CSE为0到5周，与品种（TME 12或Kibaha）或来源（SM或LL）无关。这项研究进一步得出结论，应该在第四个CSE周期后丢弃循环胚胎，并应使用新鲜的起始原料重新开始体细胞胚发生过程。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号