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首页> 外文期刊>Brazilian Oral Research >Mixed biofilms formed by C. albicans and non- albicans species: a study of microbial interactions
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Mixed biofilms formed by C. albicans and non- albicans species: a study of microbial interactions

机译：白色念珠菌和非白色念珠菌物种形成的混合生物膜：微生物相互作用的研究

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Most Candida infections are related to microbial biofilms often formed by the association of different species. The objective of this study was to evaluate the interactions between Candida albicans and non- albicans species in biofilms formed in vitro . The non- albicans species studied were: Candida tropicalis , Candida glabrata and Candida krusei . Single and mixed biofilms (formed by clinical isolates of C. albicans and non- albicans species) were developed from standardized suspensions of each strain (10 7 cells/mL), on flat-bottom 96-well microtiter plates for 48 hour. These biofilms were analyzed by counting colony-forming units (CFU/mL) in Candida HiChrome agar and by determining cell viability, using the XTT 2,3-bis (2-methoxy-4-nitro-5-sulphophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide colorimetric assay. The results for both the CFU/mL count and the XTT colorimetric assay showed that all the species studied were capable of forming high levels of in vitro biofilm. The number of CFU/mL and the metabolic activity of C. albicans were reduced in mixed biofilms with non- albicans species, as compared with a single C. albicans biofilm. Among the species tested, C. krusei exerted the highest inhibitory action against C. albicans . In conclusion, C. albicans established antagonistic interactions with non- albicans Candida species in mixed biofilms.
机译：大多数念珠菌感染与微生物生物膜有关，这些微生物生物膜通常是由不同物种的结合形成的。这项研究的目的是评估在体外形成的生物膜中白色念珠菌和非白色念珠菌之间的相互作用。研究的非白色念珠菌物种为：热带假丝酵母，光滑假丝酵母和克鲁斯假丝酵母。从每种菌株的标准悬浮液（10 7细胞/ mL）在平底96孔微量滴定板上培养48小时，形成单个和混合生物膜（由白色念珠菌和非白色念珠菌属的临床分离物形成）。使用XTT 2,3-双（2-甲氧基-4-硝基-5-硝基苯基）-5- [XTT]计数菌落Hic琼脂中的菌落形成单位（CFU / mL）并测定细胞活力，从而分析这些生物膜。 （苯氨基）羰基] -2H-氢氧化四唑比色法。 CFU / mL计数和XTT比色测定的结果表明，所有研究的物种均能够形成高水平的体外生物膜。与单个白色念珠菌生物膜相比，在混合了非白色念珠菌的生物膜中，白色念珠菌的CFU / mL数量和代谢活性降低了。在测试的物种中，克鲁斯梭菌对白色念珠菌的抑制作用最高。总之，白色念珠菌与非白色念珠菌在混合生物膜中建立了拮抗作用。

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