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首页> 外文期刊>British Biotechnology Journal >Listeria spp. in Raw Cow and Goat Meat in PortHarcourt, Nigeria
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Listeria spp. in Raw Cow and Goat Meat in PortHarcourt, Nigeria

机译：李斯特菌属尼日利亚哈科特港的生牛和山羊肉中的脂肪

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Aims: To determine the occurrence of Listeria monocytogenes and other Listeria spp. in raw cow and goat meat.Study Design: This work was based on a completely randomized design with two replication and the average values calculated for mean comparison.Place and Duration of Study: Department of Microbiology, University of Port Harcourt, Nigeria and Nigeria Institute of Medical Research, Yaba, Lagos, Nigeria. Isolation of Listeria spp. was between March 2011 and February 2012.Methodology: In this study, a total of 240 raw cow and goat meat samples were analyzed for the presence of Listeria monocytogenes and other Listeria species. The techniques recommended by the United State Department of Agriculture (USDA) revised and the Health Products and Food Methods of the Government of Canada were employed using Fraser broth and polymixin acriflavin lithium chloride ceftazidime aesculin mannitol (PALCAM) agar.Results: The results of conventional and polymerase chain reaction (PCR) characterization of the isolates revealed that 81 samples (33.75%) were positive for Listeria spp. The highest prevalence of Listeria was found in cow flesh samples (19 of 36 samples; 52.78%) followed by cow intestine samples (8 of 20; 40%) and least was goat kidney samples (5 of 28; 17.86%). Out of the 310 characterized Listeria spp., L. monocytogenes were 4(1.29%). Other species isolated were L. innocua 20(6.45%), L. ivanovii 4(1.29%), L. seeligeri 72 (23.23%), L. welshimeri 139 (44.84%) and L. grayi 71(22.90%). No L. monocyogenes was isolated from cow samples. A higher incidence was noted during the raining season 216 (69.68%) than the dry season 94(30.32%).Conclusion: This study demonstrated the occurrence and distribution of Listeria species in retail raw cow and goat meat in Port Harcourt, Nigeria.
机译：目的：确定李斯特菌和其他李斯特菌属的发生。研究设计：这项工作是基于完全随机的设计，有两次重复，并计算了平均值以进行均值比较。研究的地点和时间：尼日利亚哈科特港大学微生物学系和尼日利亚研究所尼日利亚拉各斯Yaba医学研究中心。分离李斯特菌方法是在2011年3月至2012年2月之间进行。修订了美国农业部（USDA）推荐的技术，并使用Fraser肉汤和多混菌素acriflavin氯化锂头孢他啶七叶皂苷甘露醇（PALCAM）琼脂采用了加拿大政府的《保健产品和食品方法》。结果：常规结果分离株的聚合酶链反应（PCR）表征表明81份样品（33.75％）为李斯特菌属阳性。在牛肉样品中发现李斯特菌的患病率最高（36个样品中的19个; 52.78％），其次是牛肠样品（20个中的8个; 40％），山羊肾样品中的最小（28个中的5个; 17.86％） 。在310个特征李斯特菌属中，单核细胞增生李斯特菌为4个（1.29％）。分离出的其他物种为无毒李子20（6.45％），伊万诺维氏4（1.29％），Seeligeri 72（23.23％），welshimeri 139（44.84 ％）和Grayi 71（22.90）。 ％）。没有从母牛样品中分离出单核细胞增生李斯特氏菌。在雨季216（69.68 ％）比旱季94（30.32 ％）的发病率要高。结论：本研究证明了尼日利亚哈科特港零售生牛和山羊肉中李斯特菌的发生和分布。

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