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首页> 外文期刊>British Biotechnology Journal >Response Surface Methodology Based Optimizationof a New Isolate Bacillus pumilus ZR LS S2 withFibrinolytic Activity
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Response Surface Methodology Based Optimizationof a New Isolate Bacillus pumilus ZR LS S2 withFibrinolytic Activity

机译：基于响应面方法的新型纤溶芽孢杆菌ZR LS S2的优化

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Aims: The work aims to isolate potential fibrinolytic enzyme producing isolates from various samples with the aim of developing a suitable optimization strategy using response surface methodology. Study Design: Plackett- Burman and Face centered central composite design was used.Place and Duration of Study: Department of Life Sciences, University of Calicut.Methodology: Various samples were screened using fibrin agar plates for the isolation of fibrinolytic enzyme producing bacteria and was identified by 16 s rRNA sequencing. Further the physical parameters and media components were optimized using Plackett- Burman design and face centered central composite design. Results: A novel isolate with fibrinolytic potential was isolated and was identified as Bacillus pumilus ZR LS S2. A novel media was formulated using this data, for the production of fibrinolytic enzyme, which contains peptone, casein, MgSO4 and NaCl. The isolate produced 6738.384 U/ml of enzyme in optimized conditions. Conclusion: Initial studies were performed for the production of the fibrinolytic enzyme by the novel isolate, further studies are required to effectively validate the potential of the fibrinolytic enzyme and to develop effective application for the same.
机译：目的：这项工作旨在从各种样品中分离出潜在的产生纤溶酶的分离株，目的是利用响应面方法开发一种合适的优化策略。研究设计：采用Plackett-Burman和Face为中心的中央复合设计。研究的地点和时间：卡利卡特大学生命科学系。方法：使用血纤蛋白琼脂板筛选各种样品，以分离产生纤维蛋白水解酶的细菌，通过16 s rRNA测序鉴定。此外，使用Plackett-Burman设计和面心中央复合材料设计优化了物理参数和媒体组件。结果：分离出具有纤溶潜力的新型分离株，鉴定为短小芽孢杆菌ZR LS S2。使用该数据配制了一种新型培养基，用于生产纤维蛋白溶解酶，其中包含蛋白one，酪蛋白，MgSO4和NaCl。在优化的条件下，分离物产生了6738.384 U / ml的酶。结论：已进行了有关新型分离物生产纤溶酶的初步研究，需要进一步研究以有效验证纤溶酶的潜力并开发有效的应用。

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