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首页> 外文期刊>British Biotechnology Journal >Screening and Media Optimization for EnhancingL-asparaginase Production, an Anticancer Agent,from Different Filamentous Fungi in Solid StateFermentation
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Screening and Media Optimization for EnhancingL-asparaginase Production, an Anticancer Agent,from Different Filamentous Fungi in Solid StateFermentation

机译：固态发酵不同丝状真菌抗癌剂L-天冬酰胺酶生产的筛选和培养基优化

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Aim: The aim of present study was to screen new potent fungal isolates and microorganisms possessing extracellular L-asparaginase production capacity. In addition, optimization of cultural and environmental conditions required for enzyme production will be carried out for the highest L-asparaginase producer in solid state fermentation (SSF) technique using agro-industrial residues.Study Design: Screening and physiological studies on the formation of L-asparaginase by Trichoderma viride F2 in order to obtain the optimum cultural and environmental conditions required for enzyme production.Place and Duration of Study: Department of Microbial Chemistry, Genetic Engineering and Biotechnology Division, National Research Centre (NRC), Cairo, Egypt, between July 2013 and June 2015.Methodology: Optimization of physical and nutritional parameters for enzyme production was investigated. Various locally available agro-industrial residues have been screened individually or as mixtures for L-asparaginase production. The combination of Rice husk (RH) with wheat bran (WB) (3:2) proved to be an efficient mixture for enzyme production as it gave the highest enzyme activity (71.87±3.19 U/g-ds) when compared to individual RH (66.71±2.76 U/g-ds) or WB (62.28±2.13 U/g-ds) substrates.Results: Maximal L-asparaginase production (113.43±5.11 U/g-ds) by T. viride F2 was obtained with moisture content of 75%, an inoculums size of 1 x 108 spores/ml and an initial medium pH of 5.0 when incubated at 28oC for four days. Presence of Tween 20 enhanced enzyme production by 1.19 folds. Glucose (1.0%), Casein (1.5%) and MgCl2 (0.05%) were found to be the best carbon, organic nitrogen and ion sources, respectively. Supplementation of the medium with NaNO3 (0.15%) as an inorganic nitrogen source further increased L-asparaginase production. Under these optimized conditions, L-asparaginase production by T. viride F2 was maximum with a yield of 276.5±13.4 U/g-ds in SSF, which was more than 19-fold enhancement in enzyme activity as compared to that obtained in the basal medium (SmF) (14.23±0.87 U/ml).Conclusion: The results suggest that choosing a suitable substrate coupled with optimization of different parameters can improves enzyme production markedly. Moreover, the production of L-asparaginase from a process based on RH and WB as substrates in SSF is economically attractive due to abundant substrates availability in agriculture-based countries with cheaper cost.
机译：目的：本研究的目的是筛选具有细胞外L-天冬酰胺酶生产能力的新型有效真菌分离物和微生物。此外，将对使用农业工业残留物的固态发酵（SSF）技术中最高的L-天冬酰胺酶生产商进行酶生产所需的文化和环境条件的优化研究设计：关于L形成的筛选和生理研究-木霉F2降解天冬酰胺酶，以获得酶生产所需的最佳培养和环境条件。研究地点和时间：埃及开罗国家研究中心基因工程和生物技术部微生物化学系2013年7月和2015年6月。方法：研究了用于生产酶的物理和营养参数的优化。已经单独或作为生产L-天冬酰胺酶的混合物筛选了各种当地可得的农业工业残留物。稻壳（RH）与麦麸（WB）（3：2）的组合被证明是一种高效的酶生产混合物，因为与单独的RH相比，它具有最高的酶活性（71.87±3.19 U / g-ds） （66.71±2.76 U / g-ds）或WB（62.28±2.13 U / g-ds）底物。结果：潮红三叶草F2产生的最大L-天冬酰胺酶产量为（113.43±5.11 U / g-ds）。当在28oC下孵育4天时，其含量为75％，接种量为1 x 108孢子/ ml，初始培养基pH为5.0。 Tween 20的存在将酶的产生提高了1.19倍。葡萄糖（1.0％），酪蛋白（1.5％）和氯化镁（0.05％）分别是最好的碳源，有机氮源和离子源。用NaNO3（0.15％）作为无机氮源补充培养基进一步增加了L-天冬酰胺酶的产量。在这些优化的条件下，S.SF中，T。viride F2产生的L-天冬酰胺酶产量最高，达到276.5±13.4 U / g-ds，与底物中获得的酶活性相比提高了19倍以上。结论（14.23±0.87 U / ml）。结论：选择合适的底物并优化各种参数可以显着提高酶的产量。此外，由于基于农业的国家中底物的可获得性较高且成本较低，因此基于RH和WB作为SSF中底物的工艺生产L-天冬酰胺酶在经济上具有吸引力。

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