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首页> 外文期刊>BMC Microbiology >Variations in amount of TSST-1 produced by clinical methicillin resistant Staphylococcus aureus (MRSA) isolates and allelic variation in accessory gene regulator (agr) locus
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Variations in amount of TSST-1 produced by clinical methicillin resistant Staphylococcus aureus (MRSA) isolates and allelic variation in accessory gene regulator (agr) locus

机译：临床耐甲氧西林金黄色葡萄球菌（MRSA）分离株产生的TSST-1量的变化以及辅助基因调节物（agr）位点的等位基因变化

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Background Staphylococcus aureus (S. aureus) is an important pathogen associated with both nosocomial and community-acquired infections and its pathogenicity is attributed to its potential to produce virulence factors. Since the amount of toxin produced is related to virulence, evaluating toxin production should be useful for controlling S. aureus infection. We previously found that some strains produce relatively large amounts of TSST-1; however, no reports have described the amount of TSST-1 produced by clinical isolates. Methods Amounts of TSST-1 produced by clinical methicillin resistant S. aureus (MRSA) isolates were measured by Western blotting. We determined their accessory gene regulator (agr) class by PCR and investigated whether TSST-1 production correlates with variations in the class and structure of the agr. Results We found that 75% of surveyed MRSA isolates (n = 152) possessed the tst gene and that 96.7% belonged to agr class 2. The concentrations of TSST-1 secreted into culture supernatants by 34 strains measured by Western blotting differed 170-fold. Sequencing the entire agr locus (n = 9) revealed that some had allelic variations regardless of the amount of TSST-1 produced whereas sequencing the sar, sigma factor B and the tst promoter region revealed no significant changes. Conclusion The amounts of TSST-1 produced by clinical MRSA isolates varied. The present results suggest that TSST-1 production is not directly associated with the agr structure, but is instead controlled by unknown transcriptional/translational regulatory systems, or synthesized by multiple regulatory mechanisms that are interlinked in a complex manner.
机译：背景金黄色葡萄球菌（S. aureus）是与医院感染和社区获得性感染均相关的重要病原体，其致病性归因于其产生毒力因子的潜力。由于产生的毒素量与毒力有关，因此评估毒素的产生对于控制金黄色葡萄球菌感染应该是有用的。我们先前发现，某些菌株会产生相对大量的TSST-1。然而，没有报道描述临床分离株产生的TSST-1的量。方法采用Western blotting法检测耐甲氧西林金黄色葡萄球菌（MRSA）临床分离株产生的TSST-1量。我们通过PCR确定了其辅助基因调节剂（agr）的类别，并研究了TSST-1的产生是否与agr的类别和结构的变化相关。结果我们发现被调查的MRSA分离株（n = 152）中有75％拥有tst基因，而96.7％属于agr 2类。通过Western印迹法测得的34株分泌到培养上清液中的TSST-1浓度相差170倍。对整个农业基因座进行测序（n = 9）表明，无论产生的TSST-1量如何，有些都具有等位基因变异，而对sar，sigma因子B和tst启动子区域进行测序则没有显着变化。结论临床MRSA分离株产生的TSST-1数量不同。目前的结果表明，TSST-1的产生并不与agr结构直接相关，而是由未知的转录/翻译调控系统控制，或由以复杂方式相互链接的多种调控机制合成。

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