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首页> 外文期刊>BMC Microbiology >Characterization of the expression of Salmonella Type III secretion system factor PrgI, SipA, SipB, SopE2, SpaO, and SptP in cultures and in mice
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Characterization of the expression of Salmonella Type III secretion system factor PrgI, SipA, SipB, SopE2, SpaO, and SptP in cultures and in mice

机译：沙门氏菌III型分泌系统因子PrgI，SipA，SipB，SopE2，SpaO和SptP在培养物中和小鼠中的表达特征

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Background The type III secretion systems (T3SSs) encoded by Salmonella pathogenicity island 1 and 2 (SPI-1 and SPI-2) are important for invasion of epithelial cells during development of Salmonella-associated enterocolitis and for replication in macrophages during systemic infection, respectively. In vitro studies have previously revealed hierarchical transport of different SPI-1 factors and ordered synergistic/antagonistic relationships between these proteins during Salmonella entry. These results suggest that the level and timing of the expression of these proteins dictate the consequences of bacterial infection and pathogenesis. However, the expression of these proteins has not been extensively studied in vivo, especially during the later stages of salmonellosis when the infection is established. Results In this study, we have constructed bacterial strains that contain a FLAG epitope inserted in frame to SPI-1 genes prgI, sipA, sipB, sopE2, spaO, and sptP, and investigated the expression of the tagged proteins both in vitro and in vivo during murine salmonellosis. The tagged Salmonella strains were inoculated intraperitoneally or intragastrically into mice and recovered from various organs. Our results provide direct evidence that PrgI and SipB are expressed in Salmonella colonizing the spleen and cecum of the infected animals at early and late stages of infection. Furthermore, this study demonstrates that the SpaO protein is expressed preferably in Salmonella colonizing the cecum but not the spleen and that SptP is expressed preferably in Salmonella colonizing the spleen but not the cecum. Conclusion These results suggest that Salmonella may express different SPI-1 proteins when they colonize specific tissues and that differential expression of these proteins may be important for tissue-specific aspects of bacterial pathogenesis such as gastroenterititis in the cecum and systemic infection in the spleen.
机译：背景沙门氏菌致病岛1和2（SPI-1和SPI-2）编码的III型分泌系统（T3SSs）对于沙门氏菌相关性小肠结肠炎发展过程中的上皮细胞浸润以及全身感染过程中巨噬细胞的复制非常重要。 。体外研究以前已经揭示了不同SPI-1因子的分层转运以及沙门氏菌进入期间这些蛋白之间的有序协同/拮抗关系。这些结果表明，这些蛋白质表达的水平和时间决定了细菌感染和发病机理的后果。但是，这些蛋白质的表达尚未在体内进行广泛研究，特别是在沙门氏菌病建立感染后的后期。结果在这项研究中，我们构建了包含FLAG表位的细菌菌株，该表位插入SPI-1基因prgI，sipA，sipB，sopE2，spaO和sptP的框架中，并研究了标记的蛋白在体内和体外的表达。在鼠沙门氏菌病期间。将标记的沙门氏菌菌株腹膜内或胃内接种到小鼠中，并从各种器官中回收。我们的结果提供了直接的证据，表明在感染的早期和晚期，沙门氏菌在感染动物脾脏和盲肠的沙门氏菌中表达了PrgI和SipB。此外，该研究表明SpaO蛋白优选在定居于盲肠的沙门氏菌中表达，而不在脾脏中表达，而SptP优选在定居于脾脏的沙门氏菌中表达，而不在盲肠中表达。结论这些结果表明，沙门氏菌在特定组织中定殖时可能表达不同的SPI-1蛋白，这些蛋白的差异表达对于细菌发病机理的组织特定方面（如盲肠胃肠炎和脾脏全身感染）可能很重要。

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