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首页> 外文期刊>BMC Microbiology >Staphylococcus aureus immunodominant surface antigen B is a cell-surface associated nucleic acid binding protein
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Staphylococcus aureus immunodominant surface antigen B is a cell-surface associated nucleic acid binding protein

机译：金黄色葡萄球菌免疫优势表面抗原B是细胞表面相关的核酸结合蛋白

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Background Staphylococcus aureus immunodominant surface antigen B (IsaB) elicits an immune response during septicemia and is generally classified as a virulence factor, but its biological function remains completely undefined. In an attempt to identify staphylococcal RNA-binding proteins, we designed an RNA Affinity Chromatography assay and subsequently isolated IsaB. Results Western analysis indicated that IsaB was both secreted and cell-surface associated. Gel Shift analysis confirmed the RNA binding activity but revealed that IsaB bound to any nucleic acid without sequence specificity. IsaB exhibited the highest affinity for double-stranded DNA followed by single-stranded DNA and RNA. Because extracellular DNA has been shown to play a role in biofilm formation, we investigated the biofilm-forming capacity of an isogenic isaB deletion mutant but we found that IsaB did not contribute to biofilm formation under any conditions tested. Conclusion IsaB is an extracellular nucleic acid binding protein, with little to no sequence specificity, but its role in virulence remains unclear.
机译：背景金黄色葡萄球菌的免疫优势表面抗原B（IsaB）在败血病期间引发免疫反应，通常被分类为毒力因子，但其生物学功能仍然完全不确定。为了鉴定葡萄球菌RNA结合蛋白，我们设计了一种RNA亲和色谱分析方法，并随后分离出了IsaB。结果Western分析表明IsaB既分泌又与细胞表面相关。凝胶位移分析证实了RNA结合活性，但是揭示了IsaB结合任何核酸而没有序列特异性。 IsaB对双链DNA的亲和力最高，其次是单链DNA和RNA。由于已显示细胞外DNA在生物膜形成中起作用，因此我们研究了同基因isaB缺失突变体的生物膜形成能力，但我们发现IsaB在任何测试条件下均无助于生物膜形成。结论IsaB是一种细胞外核酸结合蛋白，几乎没有序列特异性，但其在毒力中的作用尚不清楚。

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