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首页> 外文期刊>BMC Microbiology >Enterococcal surface protein Esp is not essential for cell adhesion and intestinal colonization of Enterococcus faecium in mice
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Enterococcal surface protein Esp is not essential for cell adhesion and intestinal colonization of Enterococcus faecium in mice

机译：肠球菌表面蛋白Esp对于小鼠肠屎肠球菌的细胞黏附和肠道定殖不是必需的

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Background Enterococcus faecium has globally emerged as a cause of hospital-acquired infections with high colonization rates in hospitalized patients. The enterococcal surface protein Esp, identified as a potential virulence factor, is specifically linked to nosocomial clonal lineages that are genetically distinct from indigenous E. faecium strains. To investigate whether Esp facilitates bacterial adherence and intestinal colonization of E. faecium, we used human colorectal adenocarcinoma cells (Caco-2 cells) and an experimental colonization model in mice. Results No differences in adherence to Caco-2 cells were found between an Esp expressing strain of E. faecium (E1162) and its isogenic Esp-deficient mutant (E1162Δesp). Mice, kept under ceftriaxone treatment, were inoculated orally with either E1162, E1162Δesp or both strains simultaneously. Both E1162 and E1162Δesp were able to colonize the murine intestines with high and comparable numbers. No differences were found in the contents of cecum and colon. Both E1162 and E1162Δesp were able to translocate to the mesenteric lymph nodes. Conclusion These results suggest that Esp is not essential for Caco-2 cell adherence and intestinal colonization or translocation of E. faecium in mice.
机译：背景粪便肠球菌已在全球范围内广泛出现，成为医院获得性感染的原因，住院患者的定植率很高。肠球菌表面蛋白Esp被鉴定为潜在的致病因子，与在基因上不同于原肠屎肠球菌菌株的医院克隆谱系有明确联系。为了研究Esp是否促进粪肠球菌的细菌粘附和肠道定殖，我们使用了人结肠直肠腺癌细胞（Caco-2细胞）和小鼠实验定殖模型。结果在表达Esp的粪肠球菌（E1162）与其等基因的Esp缺陷型突变体（E1162Δesp）之间未发现对Caco-2细胞的粘附性差异。口服头孢曲松治疗的小鼠同时口服E1162，E1162Δesp或两种菌株。 E1162和E1162Δesp都能够以高数量和可比数量定居在小鼠肠道。盲肠和结肠的含量没有差异。 E1162和E1162Δesp都能够移位到肠系膜淋巴结。结论这些结果表明，Esp对小鼠的Caco-2细胞粘附以及肠屎肠球菌的肠道定植或易位不是必需的。

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