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首页> 外文期刊>BMC Microbiology >Active immunization using exotoxin A confers protection against Pseudomonas aeruginosa infection in a mouse burn model
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Active immunization using exotoxin A confers protection against Pseudomonas aeruginosa infection in a mouse burn model

机译：使用外毒素A进行的主动免疫可在小鼠烧伤模型中预防铜绿假单胞菌感染

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Background Pseudomonas aeruginosa is an important cause of nosocomial infection and may lead to septicemia and death. We evaluated the immunogenicity of semi-purified exotoxin A from the bacterium in a mouse burn model. Methods The toxoid was prepared from exotoxin A taken from toxigenic strains of P. aeruginosa (PA 103). 50 mice were immunized with the toxoid, burned with hot metal and infected with 1 × 108 CFU of toxigenic strains of P. aeruginosa (experimental group); 25 non-immunized mice were also burned and infected (control group). The mortality rate and presence of any exotoxin and P. aeruginosa in the sera, liver and spleen were determined. Results In the experimental group, 2 mice died before the burns were administered and were excluded from the study. The remainder (48 mice) were challenged with a lethal dose of P. aeruginosa and followed for 70 days. 3 of these mice died. Neither P. aeruginosa nor exotoxin A was not detected in the liver, spleen or sera of the surviving mice. The protective efficacy of toxoid vaccination was therefore 93.8%. In the control group, all mice died from bacteremia and septicemia, most (80%) within 6 days, and P. aeruginosa and exotoxin A were isolated from sera, spleen and liver. Conclusion Active immunization of mice using a semi-purified exotoxin A derived from P. aeruginosa was 93.8% effective at protecting mice from subsequent P. aeruginosa infections in a mouse burn model.
机译：背景铜绿假单胞菌是医院感染的重要原因，可能导致败血症和死亡。我们评估了小鼠烧伤模型中细菌半纯化外毒素A的免疫原性。方法从铜绿假单胞菌（PA 103）的产毒菌株中提取外毒素A制备类毒素。用类毒素免疫50只小鼠，用铁水灼伤，并用1×10 8 sup铜绿假单胞菌产毒株感染（实验组）。还烧伤并感染25只未免疫的小鼠（对照组）。确定死亡率，血清，肝脏和脾脏中任何外毒素和铜绿假单胞菌的存在。结果实验组中有2只小鼠在烧伤前死亡，被排除在研究之外。其余（48只小鼠）用致死剂量的铜绿假单胞菌攻击，随后进行70天。这些小鼠中有3只死亡。在存活小鼠的肝脏，脾脏或血清中均未检出铜绿假单胞菌和外毒素A。因此，类毒素疫苗的保护效力为93.8％。在对照组中，所有小鼠都死于菌血症和败血病，大部分（80％）在6天内死亡，并且铜绿假单胞菌和外毒素A从血清，脾脏和肝脏中分离出来。结论使用半纯化的铜绿假单胞菌外毒素A主动免疫小鼠，可在小鼠烧伤模型中有效保护小鼠免受随后的铜绿假单胞菌感染。

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