...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Microbiology >Arginine deiminase pathway is far more important than urease for acid resistance and intracellular survival in Laribacter hongkongensis: a possible result of arc gene cassette duplication
【24h】

Arginine deiminase pathway is far more important than urease for acid resistance and intracellular survival in Laribacter hongkongensis: a possible result of arc gene cassette duplication

机译：精氨酸脱亚氨酶途径对脲酸杆菌的抗酸性和细胞内存活远比脲酶重要：可能是arc基因盒复制的结果

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Laribacter hongkongensis is a Gram-negative, urease-positive bacillus associated with invasive bacteremic infections in liver cirrhosis patients and fish-borne community-acquired gastroenteritis and traveler’s diarrhea. Its mechanisms of adaptation to various environmental niches and host defense evasion are largely unknown. During the process of analyzing the L. hongkongensis genome, a complete urease cassette and two adjacent arc gene cassettes were found. We hypothesize that the urease cassette and/or the arc gene cassettes are important for L. hongkongensis to survive in acidic environment and macrophages. In this study, we tested this hypothesis by constructing single, double and triple non-polar deletion mutants of the urease and two arc gene cassettes of L. hongkongensis using the conjugation-mediated gene deletion system and examining their effects in acidic environment in vitro, in macrophages and in a mouse model. Results HLHK9?ureA, HLHK9?ureC, HLHK9?ureD and HLHK9?ureE all exhibited no urease activity. HLHK9?arcA1 and HLHK9?arcA2 both exhibited arginine deiminase (ADI) activities, but HLHK9?arcA1/arcA2 double deletion mutant exhibited no ADI activity. At pH 2 and 3, survival of HLHK9?arcA1/arcA2 and HLHK9?ureA/arcA1/arcA2 were markedly decreased (p ureA was slightly decreased (p L. hongkongensis HLHK9. Survival of HLHK9?ureA/arcA1/arcA2 and HLHK9?arcA1/arcA2 in macrophages were also markedly decreased (p ureA was slightly decreased (p arcA1, arcA2 and ureA genes were all upregulated. Using a mouse model, HLHK9?ureA exhibited similar survival compared to HLHK9 after passing through the murine stomach, but survival of HLHK9?arcA1/arcA2 and HLHK9?ureA/arcA1/arcA2 were markedly reduced (p Conclusions In contrast to other important gastrointestinal tract pathogens, ADI pathway is far more important than urease for acid resistance and intracellular survival in L. hongkongensis. The gene duplication of the arc gene cassettes could be a result of their functional importance in L. hongkongensis.
机译：背景香港漆器是革兰氏阴性，脲酶阳性的芽孢杆菌，与肝硬化患者以及鱼源性社区获得性胃肠炎和旅行者的腹泻有关，侵袭性细菌感染。其适应各种环境利基和躲避东道国防御的机制在很大程度上尚不清楚。在分析香港L.ensis基因组的过程中，发现了一个完整的脲酶盒和两个相邻的arc基因盒。我们假设脲酶盒和/或arc基因盒对于香港乳杆菌在酸性环境和巨噬细胞中生存很重要。在这项研究中，我们使用缀合介导的基因删除系统构建了尿素酶的单，双和三非极性删除突变体和两个香港香arc弧基因盒，并检验了它们在酸性环境中的作用，从而验证了这一假设。在巨噬细胞和小鼠模型中。结果HLHK9？ureA，HLHK9？ureC，HLHK9？ureD和HLHK9？ureE均未显示脲酶活性。 HLHK9？arcA1和HLHK9？arcA2均具有精氨酸脱亚氨酶（ADI）活性，而HLHK9？arcA1 / arcA2双重缺失突变体则无ADI活性。在pH 2和3下，HLHK9？arcA1 / arcA2和HLHK9？ureA / arcA1 / arcA2的存活率显着降低（pureA略有降低（p。hongkongensisHLHK9。HLHK9？ureA / arcA1 / arcA2和HLHK9？ureA / arcA1和HLHK9？arcA1的存活率巨噬细胞中的/ arcA2也显着下降（p ureA略有下降（p arcA1，arcA2和ureA基因均被上调。）在小鼠模型中，HLHK9？ureA在穿过鼠胃后的存活率与HLHK9相似。 HLHK9？arcA1 / arcA2和HLHK9？ureA / arcA1 / arcA2显着减少（p结论与其他重要的胃肠道病原体相比，ADI途径对香港香。的耐酸性和细胞内存活率远比脲酶重要。弧形基因盒的出现可能是由于其在香港乳杆菌中的功能重要性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号