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首页> 外文期刊>British Journal of Pharmaceutical Research >Adherence of Irradiated Slime Producing Bacterial Pathogens to Biomaterial Surface and their Antimicrobial Susceptibility Associated with Catheter Infection in Bladder Cancer Patients
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Adherence of Irradiated Slime Producing Bacterial Pathogens to Biomaterial Surface and their Antimicrobial Susceptibility Associated with Catheter Infection in Bladder Cancer Patients

机译：膀胱癌患者辐照产生煤泥的细菌病原菌对生物材料表面的粘附及其与导管感染相关的药敏性

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Aims: To detect the prevalence of biofilm producers among Gram negative bacilli and Gram positive cocci bacterial pathogens along with their antimicrobial susceptibility pattern. Growth and adherence on catheter eluates and in the presence of antibiotics. Methodology: From laboratory of microbiology, one hundred samples (100 urinary catheters and 100 urine samples from the attached drainage bags) of bladder cancer patients collected in National Cancer Institute in Cairo, Egypt, were identified to species level. Slime production was investigated by the quantitative and qualitative methods. Qualitative method was carried out by tube method. Adherence assay and quantitation of biofilm was performed by spectrophotometric method by measuring the optical densities of stained bacterial films adherent to plastic tissue culture plates. Hydrophobicity was evaluated by adhesion to P-xylene. Identification and minimum inhibitory concentration (MICs) of 26 antimicrobial agents against gram negative and 24 against gram positive bacterial isolates were determined using microscan walk away 96 SI system. Plasmid profile analysis was carried out by plasmid isolation kit. Scanning electron microscopy studies for growth, adherence and biofilm formation. Impact of gamma irradiation at a dose level of 24.41Gy was studied. Results: From the processing of 100 samples, 98 cases were positive. Out of them 110 isolates of gram negative bacilli and 13 of gram positive cocci. They were belonging to 15 and 6 species respectively. Among them, 117 isolates showed positive results for adherence assay and biofilm/slime production. They were identified as; Escherichia coli, Klebsiella spp ., Enterobacter spp ., Acinetobacter, Proteus spp., Citrobacter, Alcaligenes, Empedobacter (104 strains) Staphylococcus spp. and Enterococcus (13 stains). The results obtained by different methods correlated well with strain to strain variation. Gamma irradiation resulted in changes in slime production and adherence ability for all the tested strains. Cell surface hydrophobicity (CSH) showed a hydrophobic reaction and these with increase in its value after irradiation in case of Escherichia coli. On the other hand, Staphylococcus epidermidis was moderate hydrophobic before irradiation changed to strictly hydrophilic after irradiation. All the slime producers showed reduced susceptibility to majority of antibiotics. They exhibited highest percentage susceptibility before and after in vitro gamma irradiation at a dose level 24.41Gy for both Amikacin and Imipenem. Scanning electron microscopy (SEM) confirmed growth and biofilm formation in the presence of catheter eluates only with halos surrounding the cells and visible erosion zones on catheter surfaces. The antimicrobial and adherence activity of Amikacin and Imipenem at the MIC level showed marked abnormalities in cells shape and size with significant reduction in adherence ability. Plasmid profile analysis of irradiated strains showed more extra-plasmid bands and / or difference in molecular weight. Conclusion: The biofilm assay strategy applied in this study may constitute a tool in biomaterial related infection and antimicrobial resistant research for further studies for biomaterial modification. Early detection of biofilm forming organisms can help in appropriate antibiotic choice.
机译：目的：检测革兰氏阴性杆菌和革兰氏阳性球菌细菌病原体中生物膜生产者的流行情况及其抗菌药敏性。导管洗脱液上和抗生素存在下的生长和粘附。方法：从微生物学实验室中，从埃及开罗国家癌症研究所收集的膀胱癌患者的一百份样本（100支导尿管和100份尿液样本从所附的引流袋中抽出）进行了鉴定。通过定量和定性方法研究了煤泥的产生。定性方法通过试管法进行。通过分光光度法通过测量粘附在塑料组织培养板上的染色细菌膜的光密度来进行生物膜的粘附测定和定量。通过与对二甲苯的粘附性来评估疏水性。使用microscan walkaway 96 SI系统确定了针对革兰氏阴性菌和24株革兰氏阳性菌的鉴定和最小抑菌浓度（MICs）。质粒谱分析通过质粒分离试剂盒进行。扫描电子显微镜研究生长，粘附和生物膜形成。研究了剂量为24.41Gy的伽马射线辐照的影响。结果：从100份样品中，有98例呈阳性。其中有110株革兰氏阴性杆菌和13株革兰氏阳性球菌。它们分别属于15和6种。其中，117株分离物显示出粘附测定和生物膜/粘液产生的阳性结果。他们被确定为;大肠杆菌，克雷伯菌属，肠杆菌属，不动杆菌属，变形杆菌属，柠檬酸杆菌，产碱杆菌，Empedobacter（104株）葡萄球菌属。和肠球菌（13个污渍）。通过不同方法获得的结果与应变之间的相关性很好。伽马辐射导致所有测试菌株的粘液产生和粘附能力发生变化。细胞表面疏水性（CSH）表现出疏水反应，在大肠杆菌的照射下，细胞表面疏水性随其值的增加而增加。另一方面，表皮葡萄球菌在照射前为中等疏水性，在照射后变为严格亲水性。所有的粘液生产商都显示出对大多数抗生素的敏感性降低。在阿米卡星和亚胺培南的剂量水平为24.41Gy的体外伽马射线辐照之前和之后，它们表现出最高的敏感性。扫描电子显微镜（SEM）证实，在存在导管洗脱液的情况下，生长和生物膜的形成仅具有围绕细胞的光晕和导管表面上可见的腐蚀区。阿米卡星和亚胺培南在MIC水平的抗菌和粘附活性显示细胞形状和大小明显异常，粘附能力显着降低。辐照菌株的质粒谱分析显示更多的质粒外谱带和/或分子量差异。结论：本研究中应用的生物膜测定策略可能构成了与生物材料相关的感染和抗菌素耐药性研究的工具，可用于进一步研究生物材料修饰。及早发现形成生物膜的生物可以帮助选择适当的抗生素。

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