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首页> 外文期刊>BMC Neuroscience >Dorsal horn-enriched genes identified by DNA microarray, in situ hybridization and immunohistochemistry
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Dorsal horn-enriched genes identified by DNA microarray, in situ hybridization and immunohistochemistry

机译：通过DNA芯片，原位杂交和免疫组织化学鉴定背角丰富的基因

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Background Neurons in the dorsal spinal cord play important roles in nociception and pain. These neurons receive input from peripheral sensory neurons and then transmit the signals to the brain, as well as receive and integrate descending control signals from the brain. Many molecules important for pain transmission have been demonstrated to be localized to the dorsal horn of the spinal cord. Further understanding of the molecular interactions and signaling pathways in the dorsal horn neurons will require a better knowledge of the molecular neuroanatomy in the dorsal spinal cord. Results A large scale screening was conducted for genes with enriched expression in the dorsal spinal cord using DNA microarray and quantitative real-time PCR. In addition to genes known to be specifically expressed in the dorsal spinal cord, other neuropeptides, receptors, ion channels, and signaling molecules were also found enriched in the dorsal spinal cord. In situ hybridization and immunohistochemistry revealed the cellular expression of a subset of these genes. The regulation of a subset of the genes was also studied in the spinal nerve ligation (SNL) neuropathic pain model. In general, we found that the genes that are enriched in the dorsal spinal cord were not among those found to be up-regulated in the spinal nerve ligation model of neuropathic pain. This study also provides a level of validation of the use of DNA microarrays in conjunction with our novel analysis algorithm (SAFER) for the identification of differences in gene expression. Conclusion This study identified molecules that are enriched in the dorsal horn of the spinal cord and provided a molecular neuroanatomy in the spinal cord, which will aid in the understanding of the molecular mechanisms important in nociception and pain.
机译：背景脊髓背神经元在伤害感受和疼痛中起重要作用。这些神经元接收来自周围感觉神经元的输入，然后将信号传输到大脑，以及接收和整合来自大脑的递减控制信号。已经证明许多对疼痛传递很重要的分子位于脊髓的背角。进一步了解背角神经元中的分子相互作用和信号传导途径将需要对背脊髓中的分子神经解剖学有更好的了解。结果利用DNA芯片和实时荧光定量PCR技术，对脊髓背侧富集基因进行了大规模筛选。除了已知在背脊髓中特异性表达的基因外，还发现其他神经肽，受体，离子通道和信号分子在背脊髓中富集。原位杂交和免疫组织化学揭示了这些基因的一个子集的细胞表达。还在脊髓神经结扎（SNL）神经性疼痛模型中研究了基因的一个子集的调节。通常，我们发现，在神经性疼痛的脊髓神经结扎模型中，富集于背脊髓中的基因不在上调基因中。这项研究还提供了对DNA微阵列与我们新颖的分析算法（SAFER）一起用于鉴定基因表达差异的验证水平。结论本研究确定了富含在脊髓背角的分子，并在脊髓中提供了分子神经解剖学，这将有助于理解对伤害感受和疼痛重要的分子机制。

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