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首页> 外文期刊>BMC Neuroscience >Separate urinary bladder and prostate neurons in the central nervous system of the rat: simultaneous labeling with two immunohistochemically distinguishable pseudorabies viruses
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Separate urinary bladder and prostate neurons in the central nervous system of the rat: simultaneous labeling with two immunohistochemically distinguishable pseudorabies viruses

机译：大鼠中枢神经系统中的单独的膀胱和前列腺神经元：用两种免疫组化可区分的假狂犬病毒同时标记

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Background This work examines the central nervous system distribution of virus-labeled neurons from the rat urinary bladder and the prostate simultaneously within the same tissue sections. Two immunohistochemically distinct pseudorabies virus strains were simultaneously injected into male Sprague Dawley rats (~280 gm). One virus was injected into the bladder and the other into the prostate. After incubation intervals of 2.25, 2.5, 2.75, 3 and 4 days, sections from the spinal cord and brain were processed immunohistochemically to detect cells, within a single section, which were labeled separately by each virus or were labeled by both viruses. Results Each strain of virus labeled a separate population of neurons and some neurons were labeled by both strains. The majority of neurons labeled by virus from the urinary bladder were found in the L6-S1 spinal cord segments within the dorsal gray commissure, the intermediolateral area and the superficial dorsal horn. Neurons labeled by virus from the prostate were mainly found in the L1-L2 spinal cord segments in the dorsal gray commissure and the intermediolateral areas. Double-labeled interneurons in L1-L2 were mainly located in the intermediolateral area. In L6-S1 they were divided between the dorsal gray commissure and the intermediolateral area. Conclusions Spinal neurons innervating the bladder are clearly separate and different from those innervating the prostate. This difference also persists in the brain. In disagreement with previous reports, no direct anatomical evidence of parasympathetic innervation of the prostate was observed.
机译：背景技术这项研究检查了来自大鼠膀胱和前列腺的病毒标记神经元同时在同一组织切片中的中枢神经系统分布。将两种免疫组化不同的伪狂犬病毒株同时注射到雄性Sprague Dawley大鼠（〜280 gm）中。一种病毒被注入膀胱，另一种病毒被注入前列腺。在2.25、2.5、2.75、3和4天的孵育间隔后，对来自脊髓和大脑的切片进行免疫组织化学处理，以检测单个切片内的细胞，这些细胞分别用每种病毒标记或用两种病毒标记。结果每种病毒株都标记了一个单独的神经元群体，并且两个株都标记了一些神经元。膀胱病毒标记的大多数神经元出现在背灰色连合，中间外侧区域和浅背角内的L6-S1脊髓节段中。前列腺病毒标记的神经元主要存在于背灰合缝和中间外侧区域的L1-L2脊髓节段。 L1-L2中的双标记中间神经元主要位于中间外侧区域。在L6-S1中，它们被分为背侧灰色连合和内侧间区域。结论支配膀胱的脊髓神经元与支配前列腺的神经元明显分离且不同。这种差异也存在于大脑中。与先前的报道不同，没有观察到前列腺副交感神经支配的直接解剖学证据。

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