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首页> 外文期刊>BMC Neuroscience >Identification of Tmem10/Opalin as a novel marker for oligodendrocytes using gene expression profiling
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Identification of Tmem10/Opalin as a novel marker for oligodendrocytes using gene expression profiling

机译：使用基因表达谱鉴定Tmem10 / Opalin作为少突胶质细胞的新标记

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Background During the development of the central nervous system, oligodendrocytes generate large amounts of myelin, a multilayered insulating membrane that ensheathes axons, thereby allowing the fast conduction of the action potential and maintaining axonal integrity. Differentiation of oligodendrocytes to myelin-forming cells requires the downregulation of RhoA GTPase activity. Results To gain insights into the molecular mechanisms of oligodendrocyte differentiation, we performed microarray expression profiling of the oligodendroglial cell line, Oli-neu, treated with the Rho kinase (ROCK) inhibitor, Y-27632 or with conditioned neuronal medium. This resulted in the identification of the transmembrane protein 10 (Tmem10/Opalin), a novel type I transmembrane protein enriched in differentiating oligodendrocytes. In primary cultures, Tmem10 was abundantly expressed in O4-positive oligodendrocytes, but not in oligodendroglial precursor cells, astrocytes, microglia or neurons. In mature oligodendrocytes Tmem10 was enriched in the rims and processes of the cells and was only found to a lesser extent in the membrane sheets. Conclusion Together, our results demonstrate that Tmem10 is a novel marker for in vitro generated oligodendrocytes.
机译：背景技术在中枢神经系统发育过程中，少突胶质细胞会产生大量的髓磷脂，髓磷脂是一种多层的绝缘膜，可刺激轴突，从而使动作电位快速传导并保持轴突的完整性。少突胶质细胞向髓鞘形成细胞的分化需要下调RhoA GTPase活性。结果为了深入了解少突胶质细胞分化的分子机制，我们对经过Rho激酶（ROCK）抑制剂Y-27632或条件神经元培养基处理的少突胶质细胞系Oli-neu进行了微阵列表达谱分析。这导致跨膜蛋白10（Tmem10 / Opalin）的鉴定，这是一种富集分化少突胶质细胞的新型I型跨膜蛋白。在原代培养中，Tmem10在O4阳性少突胶质细胞中大量表达，但在少突胶质前体细胞，星形胶质细胞，小胶质细胞或神经元中不大量表达。在成熟的少突胶质细胞中，Tmem10在细胞的边缘和过程中富集，仅在膜片中被发现程度较小。结论总之，我们的结果表明Tmem10是体外生成的少突胶质细胞的新型标记。

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