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首页> 外文期刊>BMC Neuroscience >Non-invasive quantification of brain tumor-induced astrogliosis
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Non-invasive quantification of brain tumor-induced astrogliosis

机译：脑肿瘤诱发星形胶质细胞增生的非侵入性定量

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Background CNS injury including stroke, infection, and tumor growth lead to astrogliosis, a process that involves upregulation of glial fibrillary acidic protein (GFAP) in astrocytes. However, the kinetics of astrogliosis that is related to these insults (i.e. tumor) is largely unknown. Results Using transgenic mice expressing firefly luciferase under the regulation of the GFAP promoter (GFAP-luc), we developed a model system to monitor astrogliosis upon tumor growth in a rapid, non-invasive manner. A biphasic induction of astrogliosis was observed in our xenograft model in which an early phase of activation of GFAP was associated with inflammatory response followed by a secondary, long-term upregulation of GFAP. These animals reveal GFAP activation with kinetics that is in parallel with tumor growth. Furthermore, a strong correlation between astrogliosis and tumor size was observed. Conclusions Our results suggest that non-invasive, quantitative bioluminescent imaging using GFAP-luc reporter animal is a useful tool to monitor temporal-spatial kinetics of host-mediated astrogliosis that is associated with glioma and metastatic brain tumor growth.
机译：背景中枢神经系统损伤（包括中风，感染和肿瘤生长）导致星形胶质细胞增多症，该过程涉及星形胶质细胞中神经胶质纤维酸性蛋白（GFAP）的上调。但是，与这些侮辱（即肿瘤）有关的星形胶质化的动力学在很大程度上是未知的。结果我们使用在GFAP启动子（GFAP-luc）调控下表达萤火虫荧光素酶的转基因小鼠，开发了一种模型系统，用于以快速，无创方式监测肿瘤生长后的星形胶质变。在我们的异种移植模型中观察到星形胶质增生的双相诱导，其中GFAP激活的早期与炎症反应相关，继而继发于GFAP的长期第二次上调。这些动物显示出与肿瘤生长平行的动力学中的GFAP激活。此外，观察到星形胶质细胞增生与肿瘤大小之间有很强的相关性。结论我们的结果表明，使用GFAP-luc报告基因动物进行的非侵入性定量生物发光成像是监测宿主介导的星形胶质瘤与神经胶质瘤和转移性脑瘤生长相关的时空动力学的有用工具。

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